Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit - PubMed (original) (raw)

Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit

P Westermann et al. Nucleic Acids Res. 1981.

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Abstract

In the quaternary initiation complex, eIF-2.GMPPCP.Met-tRNAf.40S ribosomal subunit, the Met-tRNAf can be cross-linked to the beta subunit of initiation factor eIF-2 as well as to ribosomal proteins S3a and S6 by treatment with the bifunctional reagent, diepoxybutane. Using 40S subunits, modified in advance with the heterobifunctional reagent, methyl-rho-azido-benzoylaminoacetimidate, Met-tRNAf is covalently bound to the same ribosomal proteins (S3a and S6) upon irradiation of the complex with ultraviolet light. Under both conditions proteins S3a and S6, together with a limited number of other ribosomal proteins, are covalently bound to 18S ribosomal RNA.

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References

    1. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4 - PubMed
    1. Arch Biochem Biophys. 1972 Sep;152(1):385-97 - PubMed
    1. FEBS Lett. 1972 Oct 1;26(1):228-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Jan;70(1):41-5 - PubMed

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