Isolation and characterization of pneumolysin-negative mutants of Streptococcus pneumoniae - PubMed (original) (raw)

Isolation and characterization of pneumolysin-negative mutants of Streptococcus pneumoniae

M K Johnson et al. Infect Immun. 1982 Aug.

Abstract

Pneumolysin-negative mutants of Streptococcus pneumoniae were isolated after mutagenesis with ethyl methane sulfonate. Though totally devoid of pneumolysin, these strains produced alpha hemolysis on blood agar when incubated aerobically. It is concluded that the alpha hemolysis typical of pneumococci is unrelated to their pneumolysin content.

PubMed Disclaimer

Similar articles

• Hydrogen Peroxide Production by Streptococcus pneumoniae Results in Alpha-hemolysis by Oxidation of Oxy-hemoglobin to Met-hemoglobin.
  McDevitt E, Khan F, Scasny A, Thompson CD, Eichenbaum Z, McDaniel LS, Vidal JE. McDevitt E, et al. mSphere. 2020 Dec 9;5(6):e01117-20. doi: 10.1128/mSphere.01117-20. mSphere. 2020. PMID: 33298575 Free PMC article.
• Pneumococci producing beta hemolysis on agar.
  Lorian V, Popoola B. Lorian V, et al. Appl Microbiol. 1972 Jul;24(1):44-7. doi: 10.1128/am.24.1.44-47.1972. Appl Microbiol. 1972. PMID: 4403496 Free PMC article.
• Streptococcus pneumoniae produces a second haemolysin that is distinct from pneumolysin.
  Canvin JR, Paton JC, Boulnois GJ, Andrew PW, Mitchell TJ. Canvin JR, et al. Microb Pathog. 1997 Mar;22(3):129-32. doi: 10.1006/mpat.1996.0098. Microb Pathog. 1997. PMID: 9075215
• Disk test for the differentiation of pneumococci from other alpha-hemolytic streptococci.
  Lorian V, Markovits G. Lorian V, et al. Appl Microbiol. 1973 Jul;26(1):116-7. doi: 10.1128/am.26.1.116-117.1973. Appl Microbiol. 1973. PMID: 4146938 Free PMC article.
• Pneumococcal beta hemolysin produced under the effect of antibiotics.
  Lorian V, Waluschka A, Popoola B. Lorian V, et al. Appl Microbiol. 1973 Feb;25(2):290-4. doi: 10.1128/am.25.2.290-294.1973. Appl Microbiol. 1973. PMID: 4121032 Free PMC article.

Cited by

• Toxicity of pneumolysin to pulmonary endothelial cells in vitro.
  Rubins JB, Duane PG, Charboneau D, Janoff EN. Rubins JB, et al. Infect Immun. 1992 May;60(5):1740-6. doi: 10.1128/iai.60.5.1740-1746.1992. Infect Immun. 1992. PMID: 1563759 Free PMC article.
• Identification of hydrogen peroxide as a Streptococcus pneumoniae toxin for rat alveolar epithelial cells.
  Duane PG, Rubins JB, Weisel HR, Janoff EN. Duane PG, et al. Infect Immun. 1993 Oct;61(10):4392-7. doi: 10.1128/iai.61.10.4392-4397.1993. Infect Immun. 1993. PMID: 8406830 Free PMC article.
• Production and purification of Streptococcus pneumoniae hemolysin (pneumolysin).
  Kanclerski K, Möllby R. Kanclerski K, et al. J Clin Microbiol. 1987 Feb;25(2):222-5. doi: 10.1128/jcm.25.2.222-225.1987. J Clin Microbiol. 1987. PMID: 3818918 Free PMC article.

References

1. 1. J Bacteriol. 1980 Nov;144(2):698-709 - PubMed
2. 1. Infect Immun. 1972 Nov;6(5):755-60 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central