Prostaglandin synthesis by macrophages requires a specific receptor-ligand interaction - PubMed (original) (raw)

Prostaglandin synthesis by macrophages requires a specific receptor-ligand interaction

C A Rouzer et al. Proc Natl Acad Sci U S A. 1980 Jul.

Abstract

The ingestion of particles by macrophages leads to the prompt induction of prostaglandin (PG) synthesis. We have now dissected the endocytic process and examined the requirements of prostaglandin E (PGE) synthesis for particle attachment, membrane interiorization, and phagosome-lysosome fusion. Macrophages that were loaded with the polyanion dextran sulfate and exhibited a greater than 99% inhibition of phagosome-lysosome fusion produced normal amounts of PGE upon challenge with zymosan. Inhibition of membrane interiorization with cytochalasin D was similarly ineffective in blocking PGE synthesis. The addition of large numbers of unmodified polystyrene latex beads, which were readily ingested by macrophages, failed to stimulate PGE synthesis. However, when macrophages were challenged with latex beads coated with immune complexes, an increased synthesis of PGE resulted. No response occurred if the complex was prepared with the F(ab')2 fragment of IgG. Similar results occurred when nonphagocytizable Sephadex beads coated with immune complexes were employed. We conclude that particle binding to the Fc receptor of the macrophage plasma membrane is a sufficient stimulus for PGE synthesis.

PubMed Disclaimer

References

    1. J Immunol. 1967 Dec;99(6):1115-20 - PubMed
    1. Ann N Y Acad Sci. 1971 Apr 30;180:123-5 - PubMed
    1. Nature. 1971 Oct 1;233(5318):336-7 - PubMed
    1. J Exp Med. 1975 Jun 1;141(6):1269-77 - PubMed
    1. Nature. 1975 Jul 3;256(5512):47-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources