Heat shock proteins are methylated in avian and mammalian cells - PubMed (original) (raw)

Heat shock proteins are methylated in avian and mammalian cells

C Wang et al. Proc Natl Acad Sci U S A. 1981 Jun.

Abstract

Exposure of chicken cells grown in tissue culture to heat shock or sodium arsenite results in a dramatic increase in the synthesis of three major polypeptides with molecular weights of 83,000 (HSP 83), 68,000 (HSP 68; referred to here as "thermin"), and 25,000 (HSP 25). Incubation of BHK-21 or HeLa cells under the same conditions results in induction of HSP 68 and a 66,000-dalton polypeptide (HSP 66). Chicken thermin is resolved by isoelectric focusing into a major acidic and a more-basic component; mammalian thermin is resolved only into one major acidic component. HSP 83 and the acidic form of thermin are highly conserved in all avian and mammalian cells examined as judged by their electrophoretic mobilities, isoelectric points, and one-dimensional peptide maps. In addition, the acidic form of thermin is indistinguishable from a protein that copurifies with brain microtubules and that remains associated with the intermediate filament-enriched Triton/KCl cytoskeletons of cells grown in tissue culture. Thermin is also a component of skeletal myofibrils. HSP 83 and thermin are methylated in cells cultured under normal growth conditions. Induction of heat shock proteins by incubation of cells in the presence of sodium arsenite results in a marked methylation of the newly synthesized thermin. Under the same experimental conditions, no significant increase in methylation of the HSP 83 is observed. HSP 25 is not methylated in untreated cells or in cells treated with sodium arsenite. These results suggest that methylation of heat shock proteins may have an important role in regulating their function.

PubMed Disclaimer

Cited by

Monoclonal antibody to intermediate filament antigen cross-reacts with higher plant cells.
Dawson PJ, Hulme JS, Lloyd CW. Dawson PJ, et al. J Cell Biol. 1985 May;100(5):1793-8. doi: 10.1083/jcb.100.5.1793. J Cell Biol. 1985. PMID: 2580847 Free PMC article.
Nonexpression of a major heat shock gene in mouse plasmacytoma MPC-11.
Aujame L, Morgan C. Aujame L, et al. Mol Cell Biol. 1985 Jul;5(7):1780-3. doi: 10.1128/mcb.5.7.1780-1783.1985. Mol Cell Biol. 1985. PMID: 4022012 Free PMC article.
Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs.
Czarnecka E, Edelman L, Schöffl F, Key JL. Czarnecka E, et al. Plant Mol Biol. 1984 Jan;3(1):45-58. doi: 10.1007/BF00023415. Plant Mol Biol. 1984. PMID: 24310259
Coprecipitation of heat shock proteins with a cell surface glycoprotein.
Hughes EN, August JT. Hughes EN, et al. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2305-9. doi: 10.1073/pnas.79.7.2305. Proc Natl Acad Sci U S A. 1982. PMID: 6954542 Free PMC article.
ADP-ribosylation of the Mr 83,000 stress-inducible and glucose-regulated protein in avian and mammalian cells: modulation by heat shock and glucose starvation.
Carlsson L, Lazarides E. Carlsson L, et al. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4664-8. doi: 10.1073/pnas.80.15.4664. Proc Natl Acad Sci U S A. 1983. PMID: 6576354 Free PMC article.

References

1. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
1. Mol Cell Biol. 1981 Apr;1(4):303-9 - PubMed
1. Biochim Biophys Acta. 1978 Jun 22;519(1):65-75 - PubMed
1. Cell. 1978 Dec;15(4):1277-86 - PubMed
1. J Cell Biol. 1979 Jan;80(1):166-82 - PubMed

Heat shock proteins are methylated in avian and mammalian cells - PubMed (original) (raw)