Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G - PubMed (original) (raw)

Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G

B M Pearse. Proc Natl Acad Sci U S A. 1982 Jan.

Abstract

Coated vesicles prepared by using isotonic gradients retain their contents. Ferritin, transferrin, and immunoglobulin G have been detected in human placental coated vesicles. Triton X-100 extraction of crude coated vesicles removes contaminating uncoated membranes, leaving coated particles and their associated proteins apparently intact; this provides an efficient means of preparing large quantities of coated vesicle proteins. The clathrin cages surround "cores" of structural protein containing polypeptides of 100,000 and 50,000 molecular weight.

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