cDNA clones coding for the heavy chain of human HLA-DR antigen - PubMed (original) (raw)

cDNA clones coding for the heavy chain of human HLA-DR antigen

J S Lee et al. Proc Natl Acad Sci U S A. 1982 Jan.

Abstract

Two cDNA clones, pDRH1 and pDRH2, containing sequences specific for human HLA-DR antigens were isolated from a bank of cDNA clones made from partially purified HLA-DR mRNA from the human lymphoblastoid cell line Maja. The clones were specific for the Mr 34,000 HLA-DR antigen glycoprotein chain. The identity of these clones was established by (i) their ability to hybridize specifically to HLA-DR mRNA in a positive selection assay; (ii) mRNA species hybridizing to the cDNA clones were expressed in B-cell but not in T-cell or fibroblast cell cultures; and (iii) a nucleotide sequence in the longer clone, pDRH2, could be translated into an amino acid sequence that is identical to the limited NH2-terminal amino acid sequence available for the purified HLA-DR antigen Mr 34,000 chain. Analysis of DNA from human, mouse, and human--mouse somatic cell hybrid lines by Southern transfer of restriction endonuclease digests indicated that the HLA-DR heavy chain is encoded in chromosome 6. This finding is compatible with the location of at least one of the HLA-D/DR heavy chain genes within the HLA region. In addition, the sequences coding for HLA-DR heavy chain appear to be present in only one or a few copies in the genome and to be relatively simple in structure.

PubMed Disclaimer

References

    1. Biochemistry. 1968 Feb;7(2):659-68 - PubMed
    1. J Natl Cancer Inst. 1969 Nov;43(5):1119-28 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Natl Cancer Inst. 1972 Sep;49(3):891-5 - PubMed
    1. Ann Hum Genet. 1973 Jan;36(3):247-66 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources