Role of coated vesicles, microfilaments, and calmodulin in receptor-mediated endocytosis by cultured B lymphoblastoid cells - PubMed (original) (raw)
Role of coated vesicles, microfilaments, and calmodulin in receptor-mediated endocytosis by cultured B lymphoblastoid cells
J L Salisbury et al. J Cell Biol. 1980 Oct.
Abstract
Cell surface receptor IgM molecules of cultured human lymlphoblastoid cells (WiL2) patch and redistribute into a cap over the Golgi region of the cell after treatment with multivalent anti-IgM antibodies. During and after the redistribution, ligand-receptor clusters are endocytosed into coated pits and coated vesicles. Morphometric analysis of the distribution of ferritin-labeled ligand at EM resolution reveals the following sequence of events in the endocytosis of cell surface IgM: (a) binding of the multivalent ligand in a diffuse cell surface distribution, (b) clustering of the ligand-receptor complexes, (c) recruitment of clathrin coats to the cytoplasmic surface of the cell membrane opposite ligand-receptor clusters, (d) assembly and (e) internalization of coated vesicles, and (f) delivery of label into a large vesicular compartment, presumably partly lysosomal. Most of the labeled ligand enters this pathway. The recruitment of clathrin coats to the membrane opposite ligand-receptor clusters is sensitive to the calmodulin-directed drug Stelazine (trifluoperazine dihydrochloride). In addition, Stelazine inhibits an alternate pathway of endocytosis that does not involve coated vesicle formation. The actin-directed drug dihydrocytochalasin B has no effect on the recruitment of clathrin to the ligand-receptor clusters and the formation of coated pits and little effect on the alternate pathway, but this drug does interfere with subsequent coated vesicle formation and it inhibits capping. Cortical microfilaments that decorate with heavy meromyosin with constant polarity are observed in association with the coated regions of the plasma membrane and with coated vesicles. SDS-polyacrylamide gel electrophoresis analysis of a coated vesicle preparation isolated from WiL2 cells demonstrates that the major polypeptides in the fraction are a 175-kdalton component that comigrates with calf brain clathrin, a 42-kdalton component that comigrates with rabbit muscle actin and a 18.5-kdalton minor component that comigrates with calmodulin as well as 110-, 70-, 55-, 36-, 30-, and 17-kdalton components. These results clarify the pathways of endocytosis in this cell and suggest functional roles for calmodulin, especially in the formation of clathrin-coated pits, and for actin microfilaments in coated vesicle formation and in capping.
Similar articles
- Endocytosis of activated receptors and clathrin-coated pit formation: deciphering the chicken or egg relationship.
Santini F, Keen JH. Santini F, et al. J Cell Biol. 1996 Mar;132(6):1025-36. doi: 10.1083/jcb.132.6.1025. J Cell Biol. 1996. PMID: 8601582 Free PMC article. - Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells.
Gottlieb TA, Ivanov IE, Adesnik M, Sabatini DD. Gottlieb TA, et al. J Cell Biol. 1993 Feb;120(3):695-710. doi: 10.1083/jcb.120.3.695. J Cell Biol. 1993. PMID: 8381123 Free PMC article. - Structurally distinct endocytic pathways for B cell receptors in B lymphocytes.
Roberts AD, Davenport TM, Dickey AM, Ahn R, Sochacki KA, Taraska JW. Roberts AD, et al. Mol Biol Cell. 2020 Dec 1;31(25):2826-2840. doi: 10.1091/mbc.E20-08-0532. Epub 2020 Oct 21. Mol Biol Cell. 2020. PMID: 33085561 Free PMC article. - Endocytosis and exocytosis: current concepts of vesicle traffic in animal cells.
Willingham MC, Pastan I. Willingham MC, et al. Int Rev Cytol. 1984;92:51-92. doi: 10.1016/s0074-7696(08)61324-8. Int Rev Cytol. 1984. PMID: 6150907 Review. - TRP Channel Trafficking.
Planells-Cases R, Ferrer-Montiel A. Planells-Cases R, et al. In: Liedtke WB, Heller S, editors. TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades. Boca Raton (FL): CRC Press/Taylor & Francis; 2007. Chapter 23. In: Liedtke WB, Heller S, editors. TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades. Boca Raton (FL): CRC Press/Taylor & Francis; 2007. Chapter 23. PMID: 21204515 Free Books & Documents. Review.
Cited by
- Potential Pathogenic Impact of Cow's Milk Consumption and Bovine Milk-Derived Exosomal MicroRNAs in Diffuse Large B-Cell Lymphoma.
Melnik BC, Stadler R, Weiskirchen R, Leitzmann C, Schmitz G. Melnik BC, et al. Int J Mol Sci. 2023 Mar 23;24(7):6102. doi: 10.3390/ijms24076102. Int J Mol Sci. 2023. PMID: 37047075 Free PMC article. Review. - PKCβII activation requires nuclear trafficking for phosphorylation and Mdm2-mediated ubiquitination.
Min X, Wang S, Zhang X, Sun N, Kim KM. Min X, et al. Life Sci Alliance. 2023 Jan 30;6(4):e202201748. doi: 10.26508/lsa.202201748. Print 2023 Apr. Life Sci Alliance. 2023. PMID: 36717249 Free PMC article. - The Ins and Outs of Antigen Uptake in B cells.
McShane AN, Malinova D. McShane AN, et al. Front Immunol. 2022 Apr 26;13:892169. doi: 10.3389/fimmu.2022.892169. eCollection 2022. Front Immunol. 2022. PMID: 35572544 Free PMC article. Review. - Impact of Endocytosis Mechanisms for the Receptors Targeted by the Currently Approved Antibody-Drug Conjugates (ADCs)-A Necessity for Future ADC Research and Development.
Hammood M, Craig AW, Leyton JV. Hammood M, et al. Pharmaceuticals (Basel). 2021 Jul 15;14(7):674. doi: 10.3390/ph14070674. Pharmaceuticals (Basel). 2021. PMID: 34358100 Free PMC article. Review. - Endophilin A2 regulates B-cell endocytosis and is required for germinal center and humoral responses.
Malinova D, Wasim L, Newman R, Martínez-Riaño A, Engels N, Tolar P. Malinova D, et al. EMBO Rep. 2021 Sep 6;22(9):e51328. doi: 10.15252/embr.202051328. Epub 2021 Jul 29. EMBO Rep. 2021. PMID: 34323351 Free PMC article.
References
- Cancer. 1968 Sep;22(3):517-24 - PubMed
- J Cell Biol. 1969 Jul;42(1):202-20 - PubMed
- J Cell Biol. 1969 Nov;43(2):312-28 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- Nat New Biol. 1971 Aug 4;232(31):153-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous