Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity - PubMed (original) (raw)

Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity

J Kurjan et al. Cell. 1980 Jul.

Abstract

Yeast strains harboring indepjendent mutations within the SUP4 tyrosine tRNA gene have been selected by virtue of their inactivating effect upon the SUP4-o UAA suppressor. Three fourths of the mutations at SUP4 are point alterations; the rest resemble the deletions described by Rothstein (1979). A meiotic genetic fine structure map of the locus was made by crossing 69 of the mutants in all combinations and testing for the frequency of SUP4-o recombinants. The sequences of SUP4 genes cloned from 32 mutant strains were determined by the dideoxynucleotide terminator method, using as primer a synthetic oligodeoxynucleotide corresponding to a sequence adjoining the SUP4 3' terminus. The positions of the DNA sequence alterations showed good colinearity with the positions of the mutations on the genetic map. One of the 26 mutant sites found by DNA sequencing lies within the intervening sequence. At this site three repeat mutations were found, each changing AT leads to TA. Whereas mutations were generally rather uniformly distributed throughout the tRNATyr coding sequence, none occurred in the DNA sequences flanking the mature tRNATyr sequence or in a 12 nucleotide sequence including the 10 bp which constitute the 3' side of the intervening sequence.

PubMed Disclaimer

Similar articles

• Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
  Goodman HM, Olson MV, Hall BD. Goodman HM, et al. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453-7. doi: 10.1073/pnas.74.12.5453. Proc Natl Acad Sci U S A. 1977. PMID: 341157 Free PMC article.
• Effects of alterations in the 3' flanking sequence on in vivo and in vitro expression of the yeast SUP4-o tRNATyr gene.
  Allison DS, Hall BD. Allison DS, et al. EMBO J. 1985 Oct;4(10):2657-64. doi: 10.1002/j.1460-2075.1985.tb03984.x. EMBO J. 1985. PMID: 3902472 Free PMC article.
• Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
  Koski RA, Clarkson SG, Kurjan J, Hall BD, Smith M. Koski RA, et al. Cell. 1980 Nov;22(2 Pt 2):415-25. doi: 10.1016/0092-8674(80)90352-9. Cell. 1980. PMID: 7004643
• Effects of altered 5'-flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene.
  Shaw KJ, Olson MV. Shaw KJ, et al. Mol Cell Biol. 1984 Apr;4(4):657-65. doi: 10.1128/mcb.4.4.657-665.1984. Mol Cell Biol. 1984. PMID: 6371493 Free PMC article.
• Development of a yeast system to assay mutational specificity.
  Pierce MK, Giroux CN, Kunz BA. Pierce MK, et al. Mutat Res. 1987 Apr;182(2):65-74. doi: 10.1016/0165-1161(87)90055-0. Mutat Res. 1987. PMID: 3550444

Cited by

• Mutations at the Saccharomyces cerevisiae SUP4 tRNA(Tyr) locus: isolation, genetic fine-structure mapping, and correlation with physical structure.
  Kurjan J, Hall BD. Kurjan J, et al. Mol Cell Biol. 1982 Dec;2(12):1501-13. doi: 10.1128/mcb.2.12.1501-1513.1982. Mol Cell Biol. 1982. PMID: 14582192 Free PMC article.
• tRNA synthesis: identification of in vivo precursor tRNAs from parental and mutant yeast strains.
  Hopper AK, Kurjan J. Hopper AK, et al. Nucleic Acids Res. 1981 Feb 25;9(4):1019-29. doi: 10.1093/nar/9.4.1019. Nucleic Acids Res. 1981. PMID: 7015284 Free PMC article.
• Pleiotropic effect of a point mutation in the yeast SUP4-o tRNA gene: in vivo pre-tRNA processing in S. cerevisiae.
  Wilhelm ML, Keith G, Fix C, Wilhelm FX. Wilhelm ML, et al. Nucleic Acids Res. 1992 Feb 25;20(4):791-6. doi: 10.1093/nar/20.4.791. Nucleic Acids Res. 1992. PMID: 1542574 Free PMC article.
• TATA-Binding protein-TATA interaction is a key determinant of differential transcription of silkworm constitutive and silk gland-specific tRNA(Ala) genes.
  Ouyang C, Martinez MJ, Young LS, Sprague KU. Ouyang C, et al. Mol Cell Biol. 2000 Feb;20(4):1329-43. doi: 10.1128/MCB.20.4.1329-1343.2000. Mol Cell Biol. 2000. PMID: 10648619 Free PMC article.
• The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.
  Munz P, Dorsch-Häsler K, Leupold U. Munz P, et al. Curr Genet. 1983 Apr;7(2):101-8. doi: 10.1007/BF00365633. Curr Genet. 1983. PMID: 24173150

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Saccharomyces Genome Database