Immunohistochemical localization of cholinergic nerve terminals - PubMed (original) (raw)
- PMID: 7020949
- DOI: 10.1007/BF00210350
Immunohistochemical localization of cholinergic nerve terminals
R T Jones et al. Cell Tissue Res. 1981.
Abstract
Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, It was used to label nerve endings in several tissues of Torpedo, rat and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.
Similar articles
- Isolation of pure cholinergic nerve endings from the electric organ of Torpedo marmorata.
Israël M, Manaranche R, Mastour-Frachon P, Morel N. Israël M, et al. Biochem J. 1976 Oct 15;160(1):113-5. doi: 10.1042/bj1600113. Biochem J. 1976. PMID: 1008840 Free PMC article. - High resolution labeling of cholinergic nerve terminals using a specific fully active biotinylated botulinum neurotoxin type A.
Arribas M, Blasi J, Egea G, Fariñas I, Solsona C, Marsal J. Arribas M, et al. J Neurosci Res. 1993 Dec 15;36(6):635-45. doi: 10.1002/jnr.490360604. J Neurosci Res. 1993. PMID: 8145292 - Quantitation of cholinergic synaptosomes from guinea pig brain.
Richardson PJ. Richardson PJ. J Neurochem. 1981 Jul;37(1):258-60. doi: 10.1111/j.1471-4159.1981.tb05319.x. J Neurochem. 1981. PMID: 6114135 - The storage and release of acetylcholine by cholinergic nerve terminals: recent results with non-mammalian preparations.
Whittaker VP, Dowdall MJ, Boyne AF. Whittaker VP, et al. Biochem Soc Symp. 1972;(36):49-68. Biochem Soc Symp. 1972. PMID: 4374951 Review. No abstract available. - Presynaptic action of snake venom neurotoxins on cholinergic systems.
Dowdall MJ, Fohlman JP, Watts A. Dowdall MJ, et al. Adv Cytopharmacol. 1979;3:63-76. Adv Cytopharmacol. 1979. PMID: 382796 Review. No abstract available.
Cited by
- Synaptic function of cholinergic-specific Chol-1alpha ganglioside.
Ando S, Tanaka Y, Kobayashi S, Fukui F, Iwamoto M, Waki H, Tai T, Hirabayashi Y. Ando S, et al. Neurochem Res. 2004 Apr;29(4):857-67. doi: 10.1023/b:nere.0000018860.75734.a7. Neurochem Res. 2004. PMID: 15098951 - Developmentally regulated O-acetylated sialoglycans in the central nervous system revealed by a new monoclonal antibody 493D4 recognizing a wide range of O-acetylated glycoconjugates.
Zhang G, Ji L, Kurono S, Fujita SC, Furuya S, Hirabayashi Y. Zhang G, et al. Glycoconj J. 1997 Nov;14(7):847-57. doi: 10.1023/a:1018542105832. Glycoconj J. 1997. PMID: 9511990 Free PMC article. - Cholinergic-specific glycoconjugates.
Whittaker VP, Kelić S. Whittaker VP, et al. Neurochem Res. 1995 Nov;20(11):1377-87. doi: 10.1007/BF00992514. Neurochem Res. 1995. PMID: 8786825 Review. - Isolation of three novel cholinergic neuron-specific gangliosides from bovine brain and their in vitro syntheses.
Irie F, Kurono S, Li YT, Seyama Y, Hirabayashi Y. Irie F, et al. Glycoconj J. 1996 Apr;13(2):177-86. doi: 10.1007/BF00731492. Glycoconj J. 1996. PMID: 8737242
References
- Biochem J. 1966 Aug;100(2):479-84 - PubMed
- J Cell Biol. 1980 Oct;87(1):104-13 - PubMed
- Naturwissenschaften. 1977 Dec;64(12):606-11 - PubMed
- Proc Natl Acad Sci U S A. 1978 Sep;75(9):4359-63 - PubMed
- Brain Res. 1974 Aug 30;77(1):85-96 - PubMed