Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals - PubMed (original) (raw)
Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals
M T Green et al. Infect Immun. 1981 Dec.
Abstract
In this study, trigeminal sensory ganglia from animals with acute herpes simplex virus, type 1 (HSV-1) infection were compared to those with a latent infection for the expression of HSV-specific antigens. By the indirect immunofluorescence assay, antisera to an immediate early polypeptide of molecular weight 175,000, designated VP175 or ICP4, and a hyperimmune antiserum to HSV-1 were used to determine whether early viral polypeptides were being expressed in neurons during the latent stage of infection. All 17 ganglia from animals with acute infection (sacrificed 3 to 12 days postinfection) exhibited positive staining when treated either with anti-HSV-1 or with anti-VP175. Forty of 42 ganglia from animals sacrificed during the latent stage of infection (22 to 200 days postinfection) exhibited immunofluorescent staining when treated with anti-VP175. The staining appeared to be similar to that observed in ganglia from acutely infected animals stained with anti-VP175, except that the number and distribution of stained cells were markedly reduced. No immunofluorescence was observed in ganglia from noninfected control animals when stained with anti-VP175 or anti-HSV-1, or when ganglia from latently infected animals were stained with anti-HSV-1 or preimmune serum.
Similar articles
- Herpes simplex virus immediate-early protein ICP4 in murine models of latency.
Pepose JS, Foos RY, Stevens JG. Pepose JS, et al. Graefes Arch Clin Exp Ophthalmol. 1986;224(4):341-5. doi: 10.1007/BF02150027. Graefes Arch Clin Exp Ophthalmol. 1986. PMID: 3011610 - The transgenic ICP4 promoter is activated in Schwann cells in trigeminal ganglia of mice latently infected with herpes simplex virus type 1.
Taus NS, Mitchell WJ. Taus NS, et al. J Virol. 2001 Nov;75(21):10401-8. doi: 10.1128/JVI.75.21.10401-10408.2001. J Virol. 2001. PMID: 11581408 Free PMC article. - Detection of HSV-1 proteins prior to the appearance of infectious virus in mouse trigeminal ganglia during reactivation of latent infection.
Wroblewska Z, Savage K, Spivack JG, Fraser NW. Wroblewska Z, et al. Virus Res. 1989 Oct;14(2):95-106. doi: 10.1016/0168-1702(89)90031-2. Virus Res. 1989. PMID: 2558462 - Immunocytochemical localization of herpes simplex virus antigen in the trigeminal ganglia of experimentally infected mice.
Itoyama Y, Sekizawa T, Openshaw H, Kogure K, Kuroiwa Y. Itoyama Y, et al. J Neurol Sci. 1984 Oct;66(1):67-75. doi: 10.1016/0022-510x(84)90142-4. J Neurol Sci. 1984. PMID: 6097651
Cited by
- Herpesvirus-bacteria synergistic interaction in periodontitis.
Chen C, Feng P, Slots J. Chen C, et al. Periodontol 2000. 2020 Feb;82(1):42-64. doi: 10.1111/prd.12311. Periodontol 2000. 2020. PMID: 31850623 Free PMC article. Review. - Herpes Simplex Virus Latency Is Noisier the Closer We Look.
Singh N, Tscharke DC. Singh N, et al. J Virol. 2020 Jan 31;94(4):e01701-19. doi: 10.1128/JVI.01701-19. Print 2020 Jan 31. J Virol. 2020. PMID: 31776275 Free PMC article. Review. - Persistent Infection with Herpes Simplex Virus 1 and Alzheimer's Disease-A Call to Study How Variability in Both Virus and Host may Impact Disease.
Mangold CA, Szpara ML. Mangold CA, et al. Viruses. 2019 Oct 20;11(10):966. doi: 10.3390/v11100966. Viruses. 2019. PMID: 31635156 Free PMC article. Review. - Lytic gene expression is frequent in HSV-1 latent infection and correlates with the engagement of a cell-intrinsic transcriptional response.
Ma JZ, Russell TA, Spelman T, Carbone FR, Tscharke DC. Ma JZ, et al. PLoS Pathog. 2014 Jul 24;10(7):e1004237. doi: 10.1371/journal.ppat.1004237. eCollection 2014 Jul. PLoS Pathog. 2014. PMID: 25058429 Free PMC article. - The herpes simplex virus type 1 latency associated transcript locus is required for the maintenance of reactivation competent latent infections.
Thompson RL, Sawtell NM. Thompson RL, et al. J Neurovirol. 2011 Dec;17(6):552-8. doi: 10.1007/s13365-011-0071-0. Epub 2011 Dec 30. J Neurovirol. 2011. PMID: 22207584 Free PMC article.
References
- JAMA. 1969 Jun 2;208(9):1675-9 - PubMed
- Science. 1971 Aug 27;173(3999):843-5 - PubMed
- Nat New Biol. 1972 Feb 16;235(59):216-7 - PubMed
- N Engl J Med. 1973 Mar 29;288(13):648-50 - PubMed
- Infect Immun. 1973 Feb;7(2):272-88 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical