Antiopsonic activity of fibrinogen bound to M protein on the surface of group A streptococci - PubMed (original) (raw)
- PMID: 7042754
- PMCID: PMC370160
- DOI: 10.1172/jci110508
Antiopsonic activity of fibrinogen bound to M protein on the surface of group A streptococci
E Whitnack et al. J Clin Invest. 1982 Apr.
Abstract
When virulent group A streptococci of M type 24 were incubated in fresh heparinized whole blood or in blood reconstituted from cellular elements and plasma, little uptake by neutrophils occurred as determined by light microscopy. When fresh human serum (with or without added heparin) was substituted for plasma, uptake occurred promptly. Uptake in serum could be prevented by adding either plasma or purified human fibrinogen to the incubation mixtures, or by pretreating the organisms with plasma or fibrinogen. Fibrinogen solutions absorbed with purified homologous M protein and centrifuged to remove precipitates lost their inhibitory activity. Uptake in serum depended on heat-labile factors. Immunofluorescent staining of bacteria using fluorescein-labeled antibody to the third component of complement showed that streptococci incubated in fresh serum bound complement evenly over the entire cell surface, whereas streptococci incubated in fresh plasma or in serum plus fibrinogen fluoresced only at some of the cross-walls between adjacent daughter cocci and at occasional terminal cocci. In electron micrographs, the surface fibrillar layer of streptococci treated with plasma or fibrinogen lost its hairlike appearance and became a dark band that stained heavily with ferritin-labeled antifibrinogen. We conclude that the known antiopsonic effect of M protein derives in part from binding of fibrinogen.
Similar articles
- Hyaluronate capsule and surface M protein in resistance to opsonization of group A streptococci.
Dale JB, Washburn RG, Marques MB, Wessels MR. Dale JB, et al. Infect Immun. 1996 May;64(5):1495-501. doi: 10.1128/iai.64.5.1495-1501.1996. Infect Immun. 1996. PMID: 8613352 Free PMC article. - Common protective antigens of group A streptococcal M proteins masked by fibrinogen.
Whitnack E, Dale JB, Beachey EH. Whitnack E, et al. J Exp Med. 1984 Apr 1;159(4):1201-12. doi: 10.1084/jem.159.4.1201. J Exp Med. 1984. PMID: 6368736 Free PMC article. - Fibrinogen binding inhibits the fixation of the third component of human complement on surface of groups A, B, C, and G streptococci.
Chhatwal GS, Dutra IS, Blobel H. Chhatwal GS, et al. Microbiol Immunol. 1985;29(10):973-80. doi: 10.1111/j.1348-0421.1985.tb02961.x. Microbiol Immunol. 1985. PMID: 3908887 - Biochemical and biological properties of the binding of human fibrinogen to M protein in group A streptococci.
Whitnack E, Beachey EH. Whitnack E, et al. J Bacteriol. 1985 Oct;164(1):350-8. doi: 10.1128/jb.164.1.350-358.1985. J Bacteriol. 1985. PMID: 3900042 Free PMC article.
Cited by
- Prominent Binding of Human and Equine Fibrinogen to Streptococcus equi subsp. zooepidemicus Is Mediated by Specific SzM Types and Is a Distinct Phenotype of Zoonotic Isolates.
Bergmann R, Jentsch MC, Uhlig A, Müller U, van der Linden M, Rasmussen M, Waller A, von Köckritz-Blickwede M, Baums CG. Bergmann R, et al. Infect Immun. 2019 Dec 17;88(1):e00559-19. doi: 10.1128/IAI.00559-19. Print 2019 Dec 17. Infect Immun. 2019. PMID: 31636136 Free PMC article. - Solution structural model of the complex of the binding regions of human plasminogen with its M-protein receptor from Streptococcus pyogenes.
Yuan Y, Ayinuola YA, Singh D, Ayinuola O, Mayfield JA, Quek A, Whisstock JC, Law RHP, Lee SW, Ploplis VA, Castellino FJ. Yuan Y, et al. J Struct Biol. 2019 Oct 1;208(1):18-29. doi: 10.1016/j.jsb.2019.07.005. Epub 2019 Jul 10. J Struct Biol. 2019. PMID: 31301349 Free PMC article. - The Molecular Basis of Human IgG-Mediated Enhancement of C4b-Binding Protein Recruitment to Group A Streptococcus.
Ermert D, Laabei M, Weckel A, Mörgelin M, Lundqvist M, Björck L, Ram S, Linse S, Blom AM. Ermert D, et al. Front Immunol. 2019 Jun 4;10:1230. doi: 10.3389/fimmu.2019.01230. eCollection 2019. Front Immunol. 2019. PMID: 31214187 Free PMC article. - Variation, Indispensability, and Masking in the M protein.
Ghosh P. Ghosh P. Trends Microbiol. 2018 Feb;26(2):132-144. doi: 10.1016/j.tim.2017.08.002. Epub 2017 Aug 31. Trends Microbiol. 2018. PMID: 28867148 Free PMC article. Review. - SCM, the M Protein of Streptococcus canis Binds Immunoglobulin G.
Bergmann S, Eichhorn I, Kohler TP, Hammerschmidt S, Goldmann O, Rohde M, Fulde M. Bergmann S, et al. Front Cell Infect Microbiol. 2017 Mar 28;7:80. doi: 10.3389/fcimb.2017.00080. eCollection 2017. Front Cell Infect Microbiol. 2017. PMID: 28401063 Free PMC article.
References
- J Exp Med. 1969 Nov 1;130(5):1063-91 - PubMed
- Infect Immun. 1977 Mar;15(3):760-4 - PubMed
- J Exp Med. 1977 Jun 1;145(6):1469-83 - PubMed
- J Infect Dis. 1979 May;139(5):575-85 - PubMed
- Acta Pathol Microbiol Scand B. 1979 Oct;87(5):303-10 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources