Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside - PubMed (original) (raw)
Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside
B Ranscht et al. Proc Natl Acad Sci U S A. 1982 Apr.
Abstract
We have generated a hybridoma cell line secreting a monoclonal antibody that specifically binds to the surfaces of oligodendrocytes and Schwann cells, the cells involved in myelin formation in the central and peripheral nervous systems, respectively. Binding studies using purified sphingolipids showed that this antibody reacts strongly with galactocerebroside (GalC), the major galactosphingolipid of myelin. The antibody was used in conjunction with rabbit antisera against sulfatide, the sulfated form of GalC, to examine the developmental appearance of these lipids on the surfaces of oligodendrocytes and Schwann cells. In addition, the loss of GalC and sulfatide from freshly dissociated Schwann cells was compared. These studied showed that GalC is expressed on the cell surface prior to sulfatide on both of these cell types in vivo and in vitro. Conversely, dissociated Schwann cells lose their cell surface sulfatide more rapidly than they lose their surface GalC under nonmyelinating conditions.
Similar articles
- Generation and biological properties of a monoclonal antibody to galactocerebroside.
Rostami A, Eccleston PA, Silberberg DH, Hirayama M, Lisak RP, Pleasure DE, Phillips SM. Rostami A, et al. Brain Res. 1984 Apr 30;298(2):203-8. doi: 10.1016/0006-8993(84)91419-7. Brain Res. 1984. PMID: 6202367 - Inhibition of in vitro peripheral myelin formation by monoclonal anti-galactocerebroside.
Ranscht B, Wood PM, Bunge RP. Ranscht B, et al. J Neurosci. 1987 Sep;7(9):2936-47. doi: 10.1523/JNEUROSCI.07-09-02936.1987. J Neurosci. 1987. PMID: 3625279 Free PMC article. - The Role of Sulfatides in Axon-Glia Interactions.
Baba H, Ishibashi T. Baba H, et al. Adv Exp Med Biol. 2019;1190:165-179. doi: 10.1007/978-981-32-9636-7_11. Adv Exp Med Biol. 2019. PMID: 31760644 Review. - Novel oligodendrocyte transmembrane signaling systems. Investigations utilizing antibodies as ligands.
Dyer CA. Dyer CA. Mol Neurobiol. 1993 Spring;7(1):1-22. doi: 10.1007/BF02780606. Mol Neurobiol. 1993. PMID: 8391270 Review.
Cited by
- Inhibition of lipid synthesis by the HIV integrase strand transfer inhibitor elvitegravir in primary rat oligodendrocyte cultures.
Monnerie H, Romer M, Roth LM, Long C, Millar JS, Jordan-Sciutto KL, Grinspan JB. Monnerie H, et al. Front Mol Neurosci. 2023 Dec 11;16:1323431. doi: 10.3389/fnmol.2023.1323431. eCollection 2023. Front Mol Neurosci. 2023. PMID: 38146334 Free PMC article. - Single-cell microglial transcriptomics during demyelination defines a microglial state required for lytic carcass clearance.
Zia S, Hammond BP, Zirngibl M, Sizov A, Baaklini CS, Panda SP, Ho MFS, Lee KV, Mainali A, Burr MK, Williams S, Caprariello AV, Power C, Simmen T, Kerr BJ, Plemel JR. Zia S, et al. Mol Neurodegener. 2022 Dec 13;17(1):82. doi: 10.1186/s13024-022-00584-2. Mol Neurodegener. 2022. PMID: 36514132 Free PMC article. - CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.
Lam M, Takeo K, Almeida RG, Cooper MH, Wu K, Iyer M, Kantarci H, Zuchero JB. Lam M, et al. Nat Commun. 2022 Sep 23;13(1):5583. doi: 10.1038/s41467-022-33200-4. Nat Commun. 2022. PMID: 36151203 Free PMC article. - Motor Learning and Physical Exercise in Adaptive Myelination and Remyelination.
Bloom MS, Orthmann-Murphy J, Grinspan JB. Bloom MS, et al. ASN Neuro. 2022 Jan-Dec;14:17590914221097510. doi: 10.1177/17590914221097510. ASN Neuro. 2022. PMID: 35635130 Free PMC article. Review. - p70S6 kinase regulates oligodendrocyte differentiation and is active in remyelinating lesions.
Benardais K, Ornelas IM, Fauveau M, Brown TL, Finseth LT, Panic R, Deboux C, Macklin WB, Wood TL, Nait-Oumesmar B. Benardais K, et al. Brain Commun. 2022 Feb 12;4(1):fcac025. doi: 10.1093/braincomms/fcac025. eCollection 2022. Brain Commun. 2022. PMID: 35224490 Free PMC article.
References
- J Neurochem. 1966 Apr;13(4):213-22 - PubMed
- J Neurochem. 1969 Apr;16(4):501-11 - PubMed
- Immunochemistry. 1971 Sep;8(9):871-4 - PubMed
- J Lipid Res. 1972 May;13(3):293-310 - PubMed
- Brain Res. 1972 Aug 25;43(2):429-35 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources