Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells - PubMed (original) (raw)

Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells

A Zumbé et al. Proc Natl Acad Sci U S A. 1982 May.

Abstract

A monoclonal antibody directed against eukaryotic mRNA 5'-cap-binding protein (anti-CBP antibody) was used to localize cap-binding protein (CBP) in BHK-21 baby hamster kidney cells by immunofluorescence microscopy. It was found that the antibody reacts with a fibrous network extending through the cytoplasm in a radial arrangement. The network behaves like intermediate filaments in colchicine-treated cells, suggesting a direct or indirect linkage of CBP with intermediate filaments. The association of CBP with a cytoskeletal element was further confirmed by isolation of proteins from Triton X-100-extracted cells and identification of CBP in the cytoskeletal fraction with anti-CBP antibody. The major polypeptide reacting with anti-CBP antibody is a Mr 50,000 component. Tryptic peptide mapping showed that this polypeptide is related to a Mr 24,000 polypeptide identified as CBP in earlier experiments [Sonenberg, N., Morgan, M. A., Testa, D., Colonna, R. J. & Shatkin, A. J. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847].

PubMed Disclaimer

Similar articles

• Association of an Mr 50,000 cap binding protein with the cytoskeleton in BHK cells.
  Trachsel H, Zumbé A, Stähli C, Hümbelin M, Sonenberg N. Trachsel H, et al. Biochem Soc Symp. 1982;47:145-61. Biochem Soc Symp. 1982. PMID: 6765493
• A 300,000-mol-wt intermediate filament-associated protein in baby hamster kidney (BHK-21) cells.
  Yang HY, Lieska N, Goldman AE, Goldman RD. Yang HY, et al. J Cell Biol. 1985 Feb;100(2):620-31. doi: 10.1083/jcb.100.2.620. J Cell Biol. 1985. PMID: 3881459 Free PMC article.
• Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells.
  Trachsel H, Sonenberg N, Shatkin AJ, Rose JK, Leong K, Bergmann JE, Gordon J, Baltimore D. Trachsel H, et al. Proc Natl Acad Sci U S A. 1980 Feb;77(2):770-4. doi: 10.1073/pnas.77.2.770. Proc Natl Acad Sci U S A. 1980. PMID: 6244584 Free PMC article.
• Probing the function of the eukaryotic 5'-cap structure using monoclonal antibodies to cap-binding proteins.
  Sonenberg N, Trachsel H. Sonenberg N, et al. Curr Top Cell Regul. 1982;21:65-88. doi: 10.1016/b978-0-12-152821-8.50007-4. Curr Top Cell Regul. 1982. PMID: 6754270 Review. No abstract available.
• The role of the cytoskeleton in eukaryotic protein synthesis. (A minireview).
  Nielsen P, Goelz S, Trachsel H. Nielsen P, et al. Cell Biol Int Rep. 1983 Apr;7(4):245-54. doi: 10.1016/0309-1651(83)90057-7. Cell Biol Int Rep. 1983. PMID: 6342818 Review. No abstract available.

Cited by

• Actin cytoskeleton in myofibroblast differentiation: ultrastructure defining form and driving function.
  Sandbo N, Dulin N. Sandbo N, et al. Transl Res. 2011 Oct;158(4):181-96. doi: 10.1016/j.trsl.2011.05.004. Epub 2011 Jun 22. Transl Res. 2011. PMID: 21925115 Free PMC article. Review.
• Emerging role for the cytoskeleton as an organizer and regulator of translation.
  Kim S, Coulombe PA. Kim S, et al. Nat Rev Mol Cell Biol. 2010 Jan;11(1):75-81. doi: 10.1038/nrm2818. Nat Rev Mol Cell Biol. 2010. PMID: 20027187 Review.
• Improper organization of the actin cytoskeleton affects protein synthesis at initiation.
  Gross SR, Kinzy TG. Gross SR, et al. Mol Cell Biol. 2007 Mar;27(5):1974-89. doi: 10.1128/MCB.00832-06. Epub 2006 Dec 18. Mol Cell Biol. 2007. PMID: 17178834 Free PMC article.
• Both microtubule-stabilizing and microtubule-destabilizing drugs inhibit hypoxia-inducible factor-1alpha accumulation and activity by disrupting microtubule function.
  Escuin D, Kline ER, Giannakakou P. Escuin D, et al. Cancer Res. 2005 Oct 1;65(19):9021-8. doi: 10.1158/0008-5472.CAN-04-4095. Cancer Res. 2005. PMID: 16204076 Free PMC article.
• A general RNA-binding protein complex that includes the cytoskeleton-associated protein MAP 1A.
  DeFranco C, Chicurel ME, Potter H. DeFranco C, et al. Mol Biol Cell. 1998 Jul;9(7):1695-708. doi: 10.1091/mbc.9.7.1695. Mol Biol Cell. 1998. PMID: 9658165 Free PMC article.

References

1. 1. J Immunol. 1965 Aug;95(2):230-45 - PubMed
2. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
3. 1. J Mol Biol. 1968 Nov 28;38(1):59-73 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 - PubMed
5. 1. Science. 1975 Mar 14;187(4180):948-50 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Miscellaneous

  • NCI CPTAC Assay Portal