Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes - PubMed (original) (raw)
Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes
P O Seglen. Biochem J. 1978.
Abstract
Protein synthesis in isolated rat hepatocytes, as measured by the incorporation of [14C]-valine at constant specific radioactivity, proceeded at a rate of 0.3-0.5%/h in an unsupplemented medium, i.e. only about one-tenth the rate of protein degradation (4%/h). Leupeptin, which inhibits lysosomal protein degradation (previously found to be 75% of the total degradation in hepatocytes), had no effect on protein synthesis, showing that endogenous protein degradation supplied amino acids in excess of the substrate requirements for protein synthesis. The inhibition of protein synthesis by NH4Cl (another inhibitor of lysosomal protein degradation) as well as the stimulation by a physiological amino acid mixture must therefore represent indirect effects, either on general energy metabolism, or on unknown regulatory processes.
Similar articles
- Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin.
Seglen PO, Grinde B, Solheim AE. Seglen PO, et al. Eur J Biochem. 1979 Apr 2;95(2):215-25. doi: 10.1111/j.1432-1033.1979.tb12956.x. Eur J Biochem. 1979. PMID: 456353 No abstract available. - Subcellular distribution of proteolytically generated valine in isolated rat hepatocytes.
Solheim AE, Seglen PO. Solheim AE, et al. Eur J Biochem. 1980 Jun;107(2):587-96. doi: 10.1111/j.1432-1033.1980.tb06067.x. Eur J Biochem. 1980. PMID: 7398655 - Differential effects of proteinase inhibitors and amines on the lysosomal and non-lysosomal pathways of protein degradation in isolated rat hepatocytes.
Grinde B, Seglen PO. Grinde B, et al. Biochim Biophys Acta. 1980 Sep 17;632(1):73-86. doi: 10.1016/0304-4165(80)90250-0. Biochim Biophys Acta. 1980. PMID: 7417519 - Protein turnover, ureagenesis and gluconeogenesis.
Schutz Y. Schutz Y. Int J Vitam Nutr Res. 2011 Mar;81(2-3):101-7. doi: 10.1024/0300-9831/a000064. Int J Vitam Nutr Res. 2011. PMID: 22139560 Review. - Methods for studying protein synthesis and degradation in liver and skeletal muscle.
Hasselgren PO, Pedersen P, Sax HC, Warner BW, Fischer JE. Hasselgren PO, et al. J Surg Res. 1988 Oct;45(4):389-415. doi: 10.1016/0022-4804(88)90136-9. J Surg Res. 1988. PMID: 3047497 Review.
Cited by
- Impaired Ribosomal Biogenesis by Noncanonical Degradation of β-Catenin during Hyperammonemia.
Davuluri G, Giusto M, Chandel R, Welch N, Alsabbagh K, Kant S, Kumar A, Kim A, Gangadhariah M, Ghosh PK, Tran U, Krajcik DM, Vasu K, DiDonato AJ, DiDonato JA, Willard B, Monga SP, Wang Y, Fox PL, Stark GR, Wessely O, Esser KA, Dasarathy S. Davuluri G, et al. Mol Cell Biol. 2019 Jul 29;39(16):e00451-18. doi: 10.1128/MCB.00451-18. Print 2019 Aug 15. Mol Cell Biol. 2019. PMID: 31138664 Free PMC article. - Influence of cell volume changes on protein synthesis in isolated hepatocytes of air-breathing walking catfish (Clarias batrachus).
Biswas K, Jyrwa LM, Häussinger D, Saha N. Biswas K, et al. Fish Physiol Biochem. 2010 Mar;36(1):17-27. doi: 10.1007/s10695-008-9275-5. Epub 2008 Nov 7. Fish Physiol Biochem. 2010. PMID: 18989741 - Effects of antibiotics on protein synthesis and degradation in primary cultures of rat hepatocytes.
Schwarze PE, Seglen PO. Schwarze PE, et al. In Vitro. 1981 Jan;17(1):71-6. doi: 10.1007/BF02618033. In Vitro. 1981. PMID: 7216241 - 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes.
Seglen PO, Gordon PB. Seglen PO, et al. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1889-92. doi: 10.1073/pnas.79.6.1889. Proc Natl Acad Sci U S A. 1982. PMID: 6952238 Free PMC article. - Cellular and lysosomal uptake of methylamine in isolated rat hepatocytes.
Solheim AE, Seglen PO. Solheim AE, et al. Biochem J. 1983 Mar 15;210(3):929-36. doi: 10.1042/bj2100929. Biochem J. 1983. PMID: 6870811 Free PMC article.
References
- Exp Cell Res. 1977 Jun;107(1):207-17 - PubMed
- Acta Biol Med Ger. 1976;35(3-4):285-99 - PubMed
- Eur J Biochem. 1978 Apr;85(1):15-25 - PubMed
- Arch Biochem Biophys. 1977 Oct;183(2):553-62 - PubMed
- Biochem J. 1977 May 15;164(2):399-407 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources