Identification of ribophorins in rough microsomal membranes from different organs of several species - PubMed (original) (raw)

Identification of ribophorins in rough microsomal membranes from different organs of several species

E E Marcantonio et al. Eur J Biochem. 1982 May.

Free article

Abstract

Microsomes prepared from several animal sources were analyzed for the presence of proteins corresponding to the ribophorins (I and II) which have been previously characterized in rat liver rough microsomes and appear to be involved in the binding of polysomes to endoplasmic reticulum membranes. In rough microsomal membranes from rat lacrimal gland, rabbit liver, dog and chicken pancreas, and mouse myeloma, ribophorin-like polypeptides with similar electrophoretic mobilities were detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. In all cases the polypeptides remained associated with sedimentable polysomes after solubilization of the microsomal membranes with nonionic detergents. Ribophorin-like polypeptides were absent from smooth microsomes. Antibodies raised against each rat liver ribophorin, purified by preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis, immunoprecipitated only the corresponding polypeptide, indicating no crossreactivity between ribophorins I and II. These antibodies also immunoprecipitated the homologous ribophorins found in microsomal preparations from other organs and species.

PubMed Disclaimer

Similar articles

• Characterization of the ribosomal binding site in rat liver rough microsomes: ribophorins I and II, two integral membrane proteins related to ribosome binding.
  Kreibich G, Czakó-Graham M, Grebenau R, Mok W, Rodriguez-Boulan E, Sabatini DD. Kreibich G, et al. J Supramol Struct. 1978;8(3):279-302. doi: 10.1002/jss.400080307. J Supramol Struct. 1978. PMID: 723266
• Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes.
  Kreibich G, Ulrich BL, Sabatini DD. Kreibich G, et al. J Cell Biol. 1978 May;77(2):464-87. doi: 10.1083/jcb.77.2.464. J Cell Biol. 1978. PMID: 649658 Free PMC article.
• The relationship between the nuclear membranes and the endoplasmic reticulum in interphase cells.
  Richardson JC, Agutter PS. Richardson JC, et al. Biochem Soc Trans. 1980 Aug;8(4):459-65. doi: 10.1042/bst0080459. Biochem Soc Trans. 1980. PMID: 7004942 Review. No abstract available.
• Isolation and structural analysis of microsomal membrane proteins.
  Heinemann FS, Ozols J. Heinemann FS, et al. Front Biosci. 1998 May 1;3:d483-93. doi: 10.2741/a295. Front Biosci. 1998. PMID: 9563973 Review.

Cited by

• The unfolded protein response in retinal vascular diseases: implications and therapeutic potential beyond protein folding.
  Zhang SX, Ma JH, Bhatta M, Fliesler SJ, Wang JJ. Zhang SX, et al. Prog Retin Eye Res. 2015 Mar;45:111-31. doi: 10.1016/j.preteyeres.2014.12.001. Epub 2014 Dec 18. Prog Retin Eye Res. 2015. PMID: 25529848 Free PMC article. Review.
• Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system.
  Khandekar M, Suzuki N, Lewton J, Yamamoto M, Engel JD. Khandekar M, et al. Mol Cell Biol. 2004 Dec;24(23):10263-76. doi: 10.1128/MCB.24.23.10263-10276.2004. Mol Cell Biol. 2004. PMID: 15542836 Free PMC article.
• DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.
  Kelleher DJ, Gilmore R. Kelleher DJ, et al. Proc Natl Acad Sci U S A. 1997 May 13;94(10):4994-9. doi: 10.1073/pnas.94.10.4994. Proc Natl Acad Sci U S A. 1997. PMID: 9144178 Free PMC article.
• Functional characterization of the cis-regulatory elements of the rat ribophorin I gene.
  Rajasekaran AK, Zhou Z, Prakash K, Das G, Kreibich G. Rajasekaran AK, et al. Nucleic Acids Res. 1995 Feb 11;23(3):313-9. doi: 10.1093/nar/23.3.313. Nucleic Acids Res. 1995. PMID: 7885824 Free PMC article.
• The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I.
  Silberstein S, Collins PG, Kelleher DJ, Rapiejko PJ, Gilmore R. Silberstein S, et al. J Cell Biol. 1995 Feb;128(4):525-36. doi: 10.1083/jcb.128.4.525. J Cell Biol. 1995. PMID: 7860628 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley