Compartmentation of mitochondrial creatine phosphokinase. I. Direct demonstration of compartmentation with the use of labeled precursors - PubMed (original) (raw)
. 1982 Dec 10;257(23):14395-404.
- PMID: 7142217
Free article
Compartmentation of mitochondrial creatine phosphokinase. I. Direct demonstration of compartmentation with the use of labeled precursors
S Erickson-Viitanen et al. J Biol Chem. 1982.
Free article
Abstract
Mitochondrial creatine kinase was first proposed to act as a functional component in respiratory control in 1966 (Bessman, S. P., and Fonyo, A. (1966) Biochem. Biophys. Res. Commun. 22, 597-602). Since that time, evidence has accumulated to support the theory of a creatine-phosphorylcreatine shuttle mechanism involved in supplying energy for aerobic muscle contraction (Bessman, S. P., and Geiger, P. J. (1981) Science 211, 448-452). To demonstrate directly the interaction between mitochondrial oxidative phosphorylation and that of creatine phosphate synthesis, we have studied the labeling of adenine nucleotides and creatine phosphate with [33P]H3PO4 or [gamma-32P]ATP over a range of adenine nucleotide concentrations incubated with rabbit cardiac and rat skeletal muscle mitochondria. An apparent direct mitochondrial ATP contribution to creatine phosphate synthesis was observed that varied inversely with the total adenine nucleotide present in the reaction system. This reaction of de novo synthesized ATP with creatine phosphokinase prior to equilibration with the total ATP pool was observed regardless of the entry point of electrons from oxidizable substrate into the electron transport chain. This special relation was not observed for added yeast hexokinase in forming glucose 6-phosphate. Mitochondria could not synthesize creatine phosphate in the presence of atractyloside, thus underscoring the requirement for adenine nucleotide translocase-linked transport of ATP prior to reaction with the bound creatine phosphokinase. These studies show that there is coupling or compartmentation of ATP synthesis and transport with creatine phosphate formation in heart and skeletal muscle mitochondria.
Similar articles
- Compartmentation of mitochondrial creatine phosphokinase. II. The importance of the outer mitochondrial membrane for mitochondrial compartmentation.
Erickson-Viitanen S, Geiger PJ, Viitanen P, Bessman SP. Erickson-Viitanen S, et al. J Biol Chem. 1982 Dec 10;257(23):14405-11. J Biol Chem. 1982. PMID: 7142218 - Studies of energy transport in heart cells. Mitochondrial isoenzyme of creatine phosphokinase: kinetic properties and regulatory action of Mg2+ ions.
Saks VA, Chernousova GB, Gukovsky DE, Smirnov VN, Chazov EI. Saks VA, et al. Eur J Biochem. 1975 Sep 1;57(1):273-90. doi: 10.1111/j.1432-1033.1975.tb02299.x. Eur J Biochem. 1975. PMID: 126157 - Transport of energy in muscle: the phosphorylcreatine shuttle.
Bessman SP, Geiger PJ. Bessman SP, et al. Science. 1981 Jan 30;211(4481):448-52. doi: 10.1126/science.6450446. Science. 1981. PMID: 6450446 Review. - Metabolic compartmentation and substrate channelling in muscle cells. Role of coupled creatine kinases in in vivo regulation of cellular respiration--a synthesis.
Saks VA, Khuchua ZA, Vasilyeva EV, Belikova OYu, Kuznetsov AV. Saks VA, et al. Mol Cell Biochem. 1994 Apr-May;133-134:155-92. doi: 10.1007/BF01267954. Mol Cell Biochem. 1994. PMID: 7808453 Review.
Cited by
- Energy metabolism design of the striated muscle cell.
Glancy B, Balaban RS. Glancy B, et al. Physiol Rev. 2021 Oct 1;101(4):1561-1607. doi: 10.1152/physrev.00040.2020. Epub 2021 Mar 18. Physiol Rev. 2021. PMID: 33733879 Free PMC article. Review. - Molecular system bioenergics of the heart: experimental studies of metabolic compartmentation and energy fluxes versus computer modeling.
Aliev M, Guzun R, Karu-Varikmaa M, Kaambre T, Wallimann T, Saks V. Aliev M, et al. Int J Mol Sci. 2011;12(12):9296-331. doi: 10.3390/ijms12129296. Epub 2011 Dec 13. Int J Mol Sci. 2011. PMID: 22272134 Free PMC article. Review. - Analyzing the functional properties of the creatine kinase system with multiscale 'sloppy' modeling.
Hettling H, van Beek JH. Hettling H, et al. PLoS Comput Biol. 2011 Aug;7(8):e1002130. doi: 10.1371/journal.pcbi.1002130. Epub 2011 Aug 11. PLoS Comput Biol. 2011. PMID: 21912519 Free PMC article. - Strong inference for systems biology.
Beard DA, Kushmerick MJ. Beard DA, et al. PLoS Comput Biol. 2009 Aug;5(8):e1000459. doi: 10.1371/journal.pcbi.1000459. Epub 2009 Aug 28. PLoS Comput Biol. 2009. PMID: 19714210 Free PMC article. No abstract available. - Effects of L-2-hydroxyglutaric acid on various parameters of the glutamatergic system in cerebral cortex of rats.
Junqueira D, Brusque AM, Porciúncula LO, Rotta LN, Ribeiro CA, Frizzo ME, Dutra Filho CS, Wannmacher CM, Wyse AT, Souza DO, Wajner M. Junqueira D, et al. Metab Brain Dis. 2003 Sep;18(3):233-43. doi: 10.1023/a:1025559200816. Metab Brain Dis. 2003. PMID: 14567473