The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the induction of interferon in human embryo fibroblasts - PubMed (original) (raw)
- PMID: 7149924
Comparative Study
The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the induction of interferon in human embryo fibroblasts
T Karpińska et al. Arch Immunol Ther Exp (Warsz). 1982.
Abstract
The influence of ultraviolet irradiation, L-ascorbic acid and calcium chloride on the production of human interferon was studied in cultures of human embryo fibroblasts (HAT). Interferon (IFN) was induced with Hertfordshire strain of Newcastle Disease Virus (NDV-H) partially inactivated with UV irradiation (NDV-Huv). The interfering properties of various NDV-Huv doses were studied in HAT tissue cultures. The highest IFN yield (IFN U per 10(6) cells) was obtained by using 100 TCID50 NDV-Huv/cell and incubating the induced cultures for 24 h at 37 degrees C. Irradiation of HAT tissue culture with UV doses of 400 and 700 erg/mm2 did not increase IFN production as expected. However, incubation of HAT tissue culture with nontoxic doses of L-ascorbic acid 10(-3) and 10(-4) M or with nontoxic doses of 1 and 4 mM calcium chloride caused an increased IFN production by over 200%.
Similar articles
- Production and initial characterization of spotted souslik (Spermophilus suslicus) interferon.
Kandefer-Szerszeń M, Kamińska T. Kandefer-Szerszeń M, et al. Arch Immunol Ther Exp (Warsz). 1984;32(4):451-7. Arch Immunol Ther Exp (Warsz). 1984. PMID: 6085464 - The influence of inhibitors of cellular synthesis and UV irradiation on interferon induction by RNA from Piptoporus betulinus.
Kandefer-Szerszeń M, Karpińska T, Kawecki Z. Kandefer-Szerszeń M, et al. Arch Immunol Ther Exp (Warsz). 1982;30(1-2):25-31. Arch Immunol Ther Exp (Warsz). 1982. PMID: 7149923 - The influence of temperature on interferon in cell cultures of homoiothermic and heterothermic mammals.
Kandefer-Szerszeń M, Kawecki Z, Meczyński S, Kaczor J. Kandefer-Szerszeń M, et al. Arch Immunol Ther Exp (Warsz). 1987;35(4):501-10. Arch Immunol Ther Exp (Warsz). 1987. PMID: 2830861 - Industrial scale production of beta-interferon.
Morandi M, Valeri A. Morandi M, et al. Adv Biochem Eng Biotechnol. 1988;37:57-72. doi: 10.1007/BFb0009177. Adv Biochem Eng Biotechnol. 1988. PMID: 3140609 Review. No abstract available.
Cited by
- Effect of high dose vitamin C on Epstein-Barr viral infection.
Mikirova N, Hunninghake R. Mikirova N, et al. Med Sci Monit. 2014 May 3;20:725-32. doi: 10.12659/MSM.890423. Med Sci Monit. 2014. PMID: 24793092 Free PMC article. - Effects of Citrus Fruit Juices and Their Bioactive Components on Inflammation and Immunity: A Narrative Review.
Miles EA, Calder PC. Miles EA, et al. Front Immunol. 2021 Jun 24;12:712608. doi: 10.3389/fimmu.2021.712608. eCollection 2021. Front Immunol. 2021. PMID: 34249019 Free PMC article. Review. - The Role and Efficacy of Vitamin C in Sepsis: A Systematic Review and Meta-Analysis.
Muhammad M, Jahangir A, Kassem A, Sattar SBA, Jahangir A, Sahra S, Niazi MRK, Mustafa A, Zia Z, Siddiqui FS, Sadiq W, Mishiyev D, Sammar A, Dahabra L, Irshad A, Elsayegh D, Chalhoub M. Muhammad M, et al. Adv Respir Med. 2022 Jul 28;90(4):281-299. doi: 10.3390/arm90040038. Adv Respir Med. 2022. PMID: 36004958 Free PMC article. - Spices, Condiments, Extra Virgin Olive Oil and Aromas as Not Only Flavorings, but Precious Allies for Our Wellbeing.
Dini I, Laneri S. Dini I, et al. Antioxidants (Basel). 2021 May 28;10(6):868. doi: 10.3390/antiox10060868. Antioxidants (Basel). 2021. PMID: 34071441 Free PMC article. Review. - Vitamin C and Immune Function.
Carr AC, Maggini S. Carr AC, et al. Nutrients. 2017 Nov 3;9(11):1211. doi: 10.3390/nu9111211. Nutrients. 2017. PMID: 29099763 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical