Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor - PubMed (original) (raw)
Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor
Y Mishima et al. Nucleic Acids Res. 1982.
Abstract
Mouse and human cell extracts (S100) can support an accurate and efficient transcription initiation on homologous ribosomal RNA gene (rDNA) templates. The cell extracts were fractionated with the aid of a phosphocellulose column into four fractions (termed A, B, C and D), including one containing a major part of the RNA polymerase I activity. Various reconstitution experiments indicate that fraction D is an absolute requirement for the correct and efficient transcription initiation by RNA polymerase I on both mouse and human genes. Fraction B effectively suppresses random initiation on these templates. Fraction A appears to further enhance the transcription which takes place with fractions C and D. Although fractions A, B and C are interchangeable between mouse and human extracts, fraction D is not; i.e. initiation of transcription required the presence of a homologous fraction D for both templates. The factor(s) in fraction D, however, is not literally species-specific, since mouse D fraction is capable of supporting accurate transcription initiation on a rat rDNA template in the presence of all the other fractions from human cell extract under the conditions where human D fraction is unable to support it. We conclude from these experiments that a species-dependent factor in fraction D plays an important role in the initiation of rDNA transcription in each animal species.
Similar articles
- A purified fraction containing RNA polymerase I that can accurately transcribe rat ribosomal RNA gene.
Kurl RN, Rothblum LI, Jacob ST. Kurl RN, et al. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6672-5. doi: 10.1073/pnas.81.21.6672. Proc Natl Acad Sci U S A. 1984. PMID: 6593724 Free PMC article. - Sequestration analysis for RNA polymerase I transcription factors with various deletion and point mutations reveals different functional regions of the mouse rRNA gene promoter.
Nagamine M, Kishimoto T, Aono J, Kato H, Kominami R, Muramatsu M. Nagamine M, et al. Mol Cell Biol. 1987 Apr;7(4):1486-95. doi: 10.1128/mcb.7.4.1486-1495.1987. Mol Cell Biol. 1987. PMID: 3600633 Free PMC article. - Rat ribosomal RNA gene can utilize primate RNA polymerase I transcription machinery: lack of absolute species specificity in rDNA transcription.
Ghosh AK, Niu H, Jacob ST. Ghosh AK, et al. Biochem Biophys Res Commun. 1996 Aug 23;225(3):890-5. doi: 10.1006/bbrc.1996.1268. Biochem Biophys Res Commun. 1996. PMID: 8780707 - Isolation, fractionation and reconstitution of a nuclear extract capable of transcribing ribosomal DNA.
Haglund RE, Rothblum LI. Haglund RE, et al. Mol Cell Biochem. 1987 Jan;73(1):11-20. doi: 10.1007/BF00229371. Mol Cell Biochem. 1987. PMID: 3807897 - Species specificity of ribosomal gene transcription: a factor associated with human RNA polymerase I prevents transcription of mouse rDNA.
Eberhard D, Grummt I. Eberhard D, et al. DNA Cell Biol. 1996 Feb;15(2):167-73. doi: 10.1089/dna.1996.15.167. DNA Cell Biol. 1996. PMID: 8634144
Cited by
- Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter.
Xu L, Li Z, Wang S. Xu L, et al. Front Microbiol. 2021 Feb 12;12:637347. doi: 10.3389/fmicb.2021.637347. eCollection 2021. Front Microbiol. 2021. PMID: 33679673 Free PMC article. - 50+ years of eukaryotic transcription: an expanding universe of factors and mechanisms.
Roeder RG. Roeder RG. Nat Struct Mol Biol. 2019 Sep;26(9):783-791. doi: 10.1038/s41594-019-0287-x. Epub 2019 Aug 22. Nat Struct Mol Biol. 2019. PMID: 31439941 Free PMC article. Review. - Structure of melon rDNA and nucleotide sequence of the 17-25S spacer region.
Kavanagh TA, Timmis JN. Kavanagh TA, et al. Theor Appl Genet. 1988 Nov;76(5):673-80. doi: 10.1007/BF00303512. Theor Appl Genet. 1988. PMID: 24232344 - Interspecies somatic cell nuclear transfer: advancements and problems.
Lagutina I, Fulka H, Lazzari G, Galli C. Lagutina I, et al. Cell Reprogram. 2013 Oct;15(5):374-84. doi: 10.1089/cell.2013.0036. Epub 2013 Sep 13. Cell Reprogram. 2013. PMID: 24033141 Free PMC article. Review. - Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.
Pikaard CS. Pikaard CS. Arabidopsis Book. 2002;1:e0083. doi: 10.1199/tab.0083. Epub 2002 Aug 12. Arabidopsis Book. 2002. PMID: 22303219 Free PMC article. No abstract available.
References
- Tohoku J Exp Med. 1966 Jan 25;88(1):69-84 - PubMed
- Anal Biochem. 1976 Dec;76(2):431-41 - PubMed
- Nucleic Acids Res. 1978 Jul;5(7):2321-32 - PubMed
- Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 - PubMed
- Proc Natl Acad Sci U S A. 1978 Nov;75(11):5367-71 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical