Effects of ethambutol on accumulation and secretion of trehalose mycolates and free mycolic acid in Mycobacterium smegmatis - PubMed (original) (raw)

Effects of ethambutol on accumulation and secretion of trehalose mycolates and free mycolic acid in Mycobacterium smegmatis

J O Kilburn et al. Antimicrob Agents Chemother. 1981 Sep.

Abstract

We examined the early effects of ethambutol on the synthesis of trehalose monomycolate, trehalose dimycolate, and free mycolic acid in actively growing cells of Mycobacterium smegmatis. At about 1 min after the addition of 3.0 micrograms of ethambutol per ml, the cellular level of trehalose monomycolate began to increase over the control culture. This was followed 8 to 12 min later by the cellular increases in free mycolic acid and trehalose dimycolate over the control culture and the inhibition of incorporation of mycolic acid into the cell wall. Exposure of M. smegmatis to ethambutol for more than 30 min caused all of these lipids to leak out of the cells more rapidly than in the control cells. The mechanism by which ethambutol initiates these events is unknown, but these early imbalances in lipid synthesis may be responsible for the lethal action of this drug.

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References

1. 1. J Gen Microbiol. 1972 Dec;73(3):539-46 - PubMed
2. 1. Antimicrob Agents Chemother. 1974 Mar;5(3):234-9 - PubMed
3. 1. Antimicrob Agents Chemother. 1981 Feb;19(2):346-8 - PubMed
4. 1. Antimicrob Agents Chemother. 1979 Aug;16(2):240-2 - PubMed
5. 1. Antimicrob Agents Chemother. 1977 May;11(5):888-96 - PubMed

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