Spontaneously active cells isolated from the sino-atrial and atrio-ventricular nodes of the rabbit heart - PubMed (original) (raw)
Spontaneously active cells isolated from the sino-atrial and atrio-ventricular nodes of the rabbit heart
J Taniguchi et al. Jpn J Physiol. 1981.
Abstract
Single cells or cell clusters composed of 3-10 cells were isolated from the S-A and A-V nodes of the rabbit heart by coronary perfusion of collagenase dissolved in Ca-free Tyrode solution (0.04%, for 1 hr). For comparison, atrial and ventricular cells were also isolated from the same heart. Shapes of the isolated nodal cell were either rod or round and nodal cells were slightly smaller than ventricular cells. Spontaneous activity was observed in both rod and round nodal cells. The action potentials had the configurations similar to those recorded from larger conventional preparations. The membrane current recorded from the small nodal cell clusters (5-10 cells) by the two-microelectrode voltage clamp technique showed a time course similar to that of previous recordings from conventional preparations, but the amplitude of the currents was 5-10 times smaller. The isolated cells showed normal sensitivities to both acetylcholine and epinephrine. Findings given in this study indicate that the isolated cells maintain the typical membrane characteristics of the nodal cells and that they are suitable for electrophysiological studies of the cardiac pacemaker cell.
Similar articles
- Action potential and membrane currents of single pacemaker cells of the rabbit heart.
Nakayama T, Kurachi Y, Noma A, Irisawa H. Nakayama T, et al. Pflugers Arch. 1984 Nov;402(3):248-57. doi: 10.1007/BF00585507. Pflugers Arch. 1984. PMID: 6097866 - Effects of verapamil on SA and AV nodal action potentials in the isolated rabbit heart.
Okada T, Konishi T. Okada T, et al. Jpn Circ J. 1975 Aug;39(8):913-7. doi: 10.1253/jcj.39.913. Jpn Circ J. 1975. PMID: 1165603 - "Fade" of hyperpolarizing responses to vagal stimulation at the sinoatrial and atrioventricular nodes of the rabbit heart.
Salata JJ, Jalife J. Salata JJ, et al. Circ Res. 1985 May;56(5):718-27. doi: 10.1161/01.res.56.5.718. Circ Res. 1985. PMID: 3995699 - [Physiology and pharmacology of the atrioventricular node].
Landmark KH. Landmark KH. Tidsskr Nor Laegeforen. 1974 Aug 10;94(21-22):1297-301. Tidsskr Nor Laegeforen. 1974. PMID: 4605860 Review. Norwegian. No abstract available. - [Current developments on the physiology of the cardiac atrial pacemaker an excitation conduction of the left atrium].
Arrigo L, Avanzino GL. Arrigo L, et al. Arch Fisiol. 1979 Jun 30;71(1-4):25-66. Arch Fisiol. 1979. PMID: 400229 Review. Italian.
Cited by
- Multiple ion channel block by the cation channel inhibitor SKF-96365 in myocytes from the rabbit atrioventricular node.
Cheng H, Curtis AE, Fellingham C, Hancox JC. Cheng H, et al. Physiol Rep. 2016 Jun;4(11):e12819. doi: 10.14814/phy2.12819. Physiol Rep. 2016. PMID: 27288059 Free PMC article. - Characterization and influence of cardiac background sodium current in the atrioventricular node.
Cheng H, Li J, James AF, Inada S, Choisy SC, Orchard CH, Zhang H, Boyett MR, Hancox JC. Cheng H, et al. J Mol Cell Cardiol. 2016 Aug;97:114-24. doi: 10.1016/j.yjmcc.2016.04.014. Epub 2016 Apr 27. J Mol Cell Cardiol. 2016. PMID: 27132017 Free PMC article. - Electrophysiological properties of myocytes isolated from the mouse atrioventricular node: L-type ICa, IKr, If, and Na-Ca exchange.
Choisy SC, Cheng H, Orchard CH, James AF, Hancox JC. Choisy SC, et al. Physiol Rep. 2015 Nov;3(11):e12633. doi: 10.14814/phy2.12633. Physiol Rep. 2015. PMID: 26607172 Free PMC article. - Cell swelling impairs dye coupling in adult rat ventricular myocytes. Cell volume as a regulator of cell communication.
De Mello WC. De Mello WC. Mol Cell Biochem. 2010 Oct;343(1-2):107-13. doi: 10.1007/s11010-010-0504-8. Epub 2010 May 30. Mol Cell Biochem. 2010. PMID: 20512611 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources