Reactivity of the thiol group in human and bovine albumin at pH 3--9, as measured by exchange with 2,2'-dithiodipyridine - PubMed (original) (raw)
Reactivity of the thiol group in human and bovine albumin at pH 3--9, as measured by exchange with 2,2'-dithiodipyridine
A O Pedersen et al. Eur J Biochem. 1980 May.
Free article
Abstract
The kinetics for exchange between an aromatic disulphide and the thiol group in human and bovine albumin as well as in glutathione were investigated in the pH range 2.5--9.8. For both albumins the rate constants exhibit a maximum near pH 3, confirming the results of Svenson and Carlsson's investigation of bovine albumin [A. Svenson and J. Carlsson (1975) Biochim. Biophys Acta, 400, 433--438]. This was related to the well known N--F conformational change of the protein. At pH 5--8 the reactivity of the thiol group in both albumins and glutathione changes sharply, probably due to ionization of the thiol group. At pH above 8, however, the reactivity of the thiol group in albumins, but not in glutathione, becomes nearly independent of pH. In addition, a conformational change at pH 6.5--8.5 was studied by means of differential spectroscopy of bilirubin, liganded to human albumin. This neutral transition appeared to proceed identically in mercaptalbumin and nonmercaptalbumin. It is concluded that (a) the pK of the thiol group in albumin is significantly below that of SH in glutathione, and (b) ionization of this thiol group, Cys-34, is independent of the neutral transition.
Similar articles
- The thiol group of bovine serum albumin. High reactivity at acidic pH as measured by the reaction with 2,2'-dipyridyl disulphide.
Svenson A, Carlsson J. Svenson A, et al. Biochim Biophys Acta. 1975 Aug 19;400(2):433-8. doi: 10.1016/0005-2795(75)90199-3. Biochim Biophys Acta. 1975. PMID: 240419
Cited by
- Molecular determinants of acrylamide neurotoxicity through covalent docking.
Mueller NPF, Carloni P, Alfonso-Prieto M. Mueller NPF, et al. Front Pharmacol. 2023 Mar 2;14:1125871. doi: 10.3389/fphar.2023.1125871. eCollection 2023. Front Pharmacol. 2023. PMID: 36937867 Free PMC article. - Comparative cisplatin reactivity towards human Zn7-metallothionein-2 and MTF-1 zinc fingers: potential implications in anticancer drug resistance.
Bulathge AW, Villones RLE, Herbert FC, Gassensmith JJ, Meloni G. Bulathge AW, et al. Metallomics. 2022 Sep 15;14(9):mfac061. doi: 10.1093/mtomcs/mfac061. Metallomics. 2022. PMID: 36026541 Free PMC article. - Staphylococcus aureus Small RNAs Possess Dephospho-CoA 5'-Caps, but No CoAlation Marks.
Löcherer C, Bühler N, Lafrenz P, Jäschke A. Löcherer C, et al. Noncoding RNA. 2022 Jun 28;8(4):46. doi: 10.3390/ncrna8040046. Noncoding RNA. 2022. PMID: 35893229 Free PMC article. - Metal binding and interdomain thermodynamics of mammalian metallothionein-3: enthalpically favoured Cu+ supplants entropically favoured Zn2+ to form Cu4 + clusters under physiological conditions.
Mehlenbacher MR, Elsiesy R, Lakha R, Villones RLE, Orman M, Vizcarra CL, Meloni G, Wilcox DE, Austin RN. Mehlenbacher MR, et al. Chem Sci. 2022 Apr 4;13(18):5289-5304. doi: 10.1039/d2sc00676f. eCollection 2022 May 11. Chem Sci. 2022. PMID: 35655557 Free PMC article. - Interspecies differences in stability kinetics and plasma esterases involved in hydrolytic activation of curcumin diethyl disuccinate, a prodrug of curcumin.
Ratnatilaka Na Bhuket P, Jithavech P, Ongpipattanakul B, Rojsitthisak P. Ratnatilaka Na Bhuket P, et al. RSC Adv. 2019 Feb 6;9(8):4626-4634. doi: 10.1039/c8ra08594c. eCollection 2019 Jan 30. RSC Adv. 2019. PMID: 35520191 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources