A screening method for isolating DNA repair-deficient mutants of CHO cells - PubMed (original) (raw)
- PMID: 7404270
- DOI: 10.1007/BF01542791
A screening method for isolating DNA repair-deficient mutants of CHO cells
L H Thompson et al. Somatic Cell Genet. 1980 May.
Abstract
A simple procedure for isolating mutagen-sensitive clones of CHO cells was developed and applied in mutant hunts in which colonies were screened for hypersensitivity to killing by ultraviolet radiation (UV, ethyl methanesulfonate (EMS), or mitomycin C (MMC). Each of two UV-sensitive clones studied in detail had a D37 dose of 1.0 J/m2 compared to 7.0 J/m2 for the wild-type cells, and each was shown to have no detectable repair replication following exposure to UV doses of up to 26 J/m2. Although these mutants resemble xeroderma pigmentosum human mutants with respect to their repair defect and cross-sensitivity to the carcinogen 4-nitroquinoline-1-oxide, one of two clones (UV-20) is characterized by extreme hypersensitivity to MMC (80-fold as compared to the wild type). Clones having hypersensitivity to alkylating agents, but not UV, were obtained using MMC and EMS. In the latter case the two clones had significantly increased sensitivity to the killing action of 60Co gamma-rays.
Similar articles
- Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells.
Jones NJ, Cox R, Thacker J. Jones NJ, et al. Mutat Res. 1987 May;183(3):279-86. doi: 10.1016/0167-8817(87)90011-3. Mutat Res. 1987. PMID: 3106801 - Large-scale isolation of UV-sensitive clones of CHO cells.
Busch DB, Cleaver JE, Glaser DA. Busch DB, et al. Somatic Cell Genet. 1980 May;6(3):407-18. doi: 10.1007/BF01542792. Somatic Cell Genet. 1980. PMID: 7404271 - Sensitivity to DNA-damaging agents and mutation induction by UV light in UV-sensitive CHO cells.
Stefanini M, Mondello C, Tessera L, Capuano V, Guerra BR, Nuzzo F. Stefanini M, et al. Mutat Res. 1986 Jun;174(2):155-9. doi: 10.1016/0165-7992(86)90108-9. Mutat Res. 1986. PMID: 3086715 - [Postreplicative DNA repair in UV-sensitive Chinese hamster cell clones].
Pinto RI, Manukian KL, Vikhanskaia FL, Manuilova ES, Shapiro NI. Pinto RI, et al. Tsitologiia. 1980 Sep;22(9):1085-95. Tsitologiia. 1980. PMID: 7003862 Review. Russian.
Cited by
- Role of Human N-Acetyltransferase 2 Genetic Polymorphism on Aromatic Amine Carcinogen-Induced DNA Damage and Mutagenicity in a Chinese Hamster Ovary Cell Mutation Assay.
Baldauf KJ, Salazar-González RA, Doll MA, Pierce WM Jr, States JC, Hein DW. Baldauf KJ, et al. Environ Mol Mutagen. 2020 Feb;61(2):235-245. doi: 10.1002/em.22331. Epub 2019 Sep 30. Environ Mol Mutagen. 2020. PMID: 31490564 Free PMC article. - Coordination of DNA single strand break repair.
Abbotts R, Wilson DM 3rd. Abbotts R, et al. Free Radic Biol Med. 2017 Jun;107:228-244. doi: 10.1016/j.freeradbiomed.2016.11.039. Epub 2016 Nov 24. Free Radic Biol Med. 2017. PMID: 27890643 Free PMC article. Review. - Global contributions to the understanding of DNA repair and skin cancer.
Kraemer KH, DiGiovanna JJ. Kraemer KH, et al. J Invest Dermatol. 2014 Oct 10;134(e1):E8-17. doi: 10.1038/skinbio.2014.3. J Invest Dermatol. 2014. PMID: 25302472 Free PMC article. Review. No abstract available. - Intracellular generation of ROS by 3,5-dimethylaminophenol: persistence, cellular response, and impact of molecular toxicity.
Chao MW, Erkekoglu P, Tseng CY, Ye W, Trudel LJ, Skipper PL, Tannenbaum SR, Wogan GN. Chao MW, et al. Toxicol Sci. 2014 Sep;141(1):300-13. doi: 10.1093/toxsci/kfu127. Epub 2014 Jun 27. Toxicol Sci. 2014. PMID: 24973092 Free PMC article. - Identification of potentially cytotoxic lesions induced by UVA photoactivation of DNA 4-thiothymidine in human cells.
Reelfs O, Macpherson P, Ren X, Xu YZ, Karran P, Young AR. Reelfs O, et al. Nucleic Acids Res. 2011 Dec;39(22):9620-32. doi: 10.1093/nar/gkr674. Epub 2011 Sep 2. Nucleic Acids Res. 2011. PMID: 21890905 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials