Pyrimidine-specific chemical reactions useful for DNA sequencing - PubMed (original) (raw)

Pyrimidine-specific chemical reactions useful for DNA sequencing

C M Rubin et al. Nucleic Acids Res. 1980.

Free PMC article

Abstract

Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).

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References

1. 1. Nucleic Acids Res. 1978 Feb;5(2):615-22 - PubMed
2. 1. Nature. 1980 Mar 27;284(5754):372-4 - PubMed
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