Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates - PubMed (original) (raw)
Comparative Study
. 1980 Mar 11;8(5):1081-96.
doi: 10.1093/nar/8.5.1081.
- PMID: 7443540
- PMCID: PMC323974
- DOI: 10.1093/nar/8.5.1081
Free PMC article
Comparative Study
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates
M J Gait et al. Nucleic Acids Res. 1980.
Free PMC article
Abstract
A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.
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