Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27 - PubMed (original) (raw)

Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27

R A Houghten et al. Int J Pept Protein Res. 1980 Oct.

Abstract

Three tritiated analogs of human beta-endorphin have been prepared from their corresponding iodinated analogs. The iodinated analogs (diiodotyrosine at positions 1, 27 or 1 and 27) were synthesized by the solid-phase method and were found to have biological and physical properties which were altered when compared with the native molecule. Catalytic exchange of these iodinated analogs in the presence of tritium yielded tritiated human beta-endorphins having full biological activity and specific activity of 50-100 Ci/mmol. Both the iodinated and tritiated beta-endorphin analogs were shown to be homogeneous by chromatography on carboxymethylcellulose, partition chromatography, paper chromatography, amino acid analysis, electrophoresis, high performance liquid chromatography, and isoelectric focusing on polyacrylamide.

PubMed Disclaimer

Cited by

Analysis of a subclass-restricted HIV-1 gp41 epitope by omission peptides.
Mathiesen T, Chiodi F, Broliden PA, Albert J, Houghten RA, Utter G, Wahren B, Norrby E. Mathiesen T, et al. Immunology. 1989 May;67(1):1-7. Immunology. 1989. PMID: 2472353 Free PMC article.
Preparation of [125I-Tyr27,Leu5]beta h-endorphin and its use for crosslinking of opioid binding sites in human striatum and NG108-15 neuroblastoma-glioma cells.
Helmeste DM, Hammonds RG Jr, Li CH. Helmeste DM, et al. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4622-5. doi: 10.1073/pnas.83.13.4622. Proc Natl Acad Sci U S A. 1986. PMID: 3014499 Free PMC article.
beta-Endorphin: characteristics of binding sites in a neuroblastoma--glioma hybrid cell.
Hammonds RG Jr, Ferrara P, Li CH. Hammonds RG Jr, et al. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2218-20. doi: 10.1073/pnas.78.4.2218. Proc Natl Acad Sci U S A. 1981. PMID: 6264469 Free PMC article.
Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2.
Altman A, Cardenas JM, Houghten RA, Dixon FJ, Theofilopoulos AN. Altman A, et al. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2176-80. doi: 10.1073/pnas.81.7.2176. Proc Natl Acad Sci U S A. 1984. PMID: 6371810 Free PMC article.
Anti-HLA antibodies of predetermined specificity: a chemically synthesized peptide induces antibodies specific for HLA-A,B heavy chain.
Church WR, Walker LE, Houghten RA, Reisfeld RA. Church WR, et al. Proc Natl Acad Sci U S A. 1983 Jan;80(1):255-8. doi: 10.1073/pnas.80.1.255. Proc Natl Acad Sci U S A. 1983. PMID: 6571999 Free PMC article.

Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27 - PubMed (original) (raw)