Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method - PubMed (original) (raw)

Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method

K Miyoshi et al. Nucleic Acids Res. 1980.

Free PMC article

Abstract

Synthesis of two oligothymidylic acids, tridecamer and nonadecamer, is described by a rapid and simple solid-phase method on two kinds of polyacrylamide supports derivatized from commercially available Enzacryl Gel K-2. The syntheses were performed by the phosphotriester method using di- and tri-thymidylic acid blocks as the incoming 3'-phosphodiester component. High coupling yields were consistently obtained and the final product was isolated very easily by high performance liquid chromatography on Permaphase AAX.

PubMed Disclaimer

Cited by

Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.
Pon RT, Yu S. Pon RT, et al. Nucleic Acids Res. 1997 Sep 15;25(18):3629-35. doi: 10.1093/nar/25.18.3629. Nucleic Acids Res. 1997. PMID: 9278483 Free PMC article.
Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.
Miyoshi K, Arentzen R, Huang T, Itakura K. Miyoshi K, et al. Nucleic Acids Res. 1980 Nov 25;8(22):5507-17. doi: 10.1093/nar/8.22.5507. Nucleic Acids Res. 1980. PMID: 7465420 Free PMC article.
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.
Miyoshi K, Huang T, Itakura K. Miyoshi K, et al. Nucleic Acids Res. 1980 Nov 25;8(22):5491-505. doi: 10.1093/nar/8.22.5491. Nucleic Acids Res. 1980. PMID: 7465419 Free PMC article.
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K. Wallace RB, et al. Nucleic Acids Res. 1981 Feb 25;9(4):879-94. doi: 10.1093/nar/9.4.879. Nucleic Acids Res. 1981. PMID: 7232206 Free PMC article.
A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.
Ohtsuka E, Tozuka Z, Iwai S, Ikehara M. Ohtsuka E, et al. Nucleic Acids Res. 1982 Oct 25;10(20):6235-41. doi: 10.1093/nar/10.20.6235. Nucleic Acids Res. 1982. PMID: 7177845 Free PMC article.

References

1. Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
1. Nucleic Acids Res. 1975 Jun;2(6):773-86 - PubMed
1. Nucleic Acids Res. 1977 Feb;4(2):353-71 - PubMed
1. J Chromatogr. 1977 Jan 21;131:169-77 - PubMed
1. Nucleic Acids Res. 1977 Apr;4(4):1047-63 - PubMed

Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method - PubMed (original) (raw)