N-glycans as apical sorting signals in epithelial cells - PubMed (original) (raw)
. 1995 Nov 2;378(6552):96-8.
doi: 10.1038/378096a0.
Affiliations
- PMID: 7477300
- DOI: 10.1038/378096a0
N-glycans as apical sorting signals in epithelial cells
P Scheiffele et al. Nature. 1995.
Abstract
In epithelial Madin-Darby canine kidney (MDCK) cells newly synthesized molecules are sorted in the trans-Golgi network and directly delivered to their apical and basolateral surface destinations. Sorting is mediated by signals in the cytoplasmic domains of basolateral transmembrane proteins whereas glycosylphosphatidylinositol-linked proteins have apical sorting information in their glycolipid tails. Signals for apical transmembrane proteins are thought to reside in their ectodomains, because truncated forms lacking the cytoplasmic tail and the membrane anchor are secreted apically. Here we demonstrate that carbohydrates act as an apical targeting signal for secretory proteins. Growth hormone, which is non-glycosylated and secreted from both sides of MDCK cell layers, is secreted from the apical side when glycosylated. Thus glycans not only play a general role in protein folding but also appear to function in protein sorting in biosynthetic traffic.
Similar articles
- N-glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells.
Pang S, Urquhart P, Hooper NM. Pang S, et al. J Cell Sci. 2004 Oct 1;117(Pt 21):5079-86. doi: 10.1242/jcs.01386. J Cell Sci. 2004. PMID: 15456847 - N-glycans mediate apical recycling of the sialomucin endolyn in polarized MDCK cells.
Potter BA, Weixel KM, Bruns JR, Ihrke G, Weisz OA. Potter BA, et al. Traffic. 2006 Feb;7(2):146-54. doi: 10.1111/j.1600-0854.2005.00371.x. Traffic. 2006. PMID: 16420523 - N-Glycan synthesis in the apical and basolateral secretory pathway of epithelial MDCK cells and the influence of a glycosaminoglycan domain.
Moen A, Hafte TT, Tveit H, Egge-Jacobsen W, Prydz K. Moen A, et al. Glycobiology. 2011 Nov;21(11):1416-25. doi: 10.1093/glycob/cwr069. Epub 2011 Jun 14. Glycobiology. 2011. PMID: 21673010 - Polarized biosynthetic traffic in renal epithelial cells: sorting, sorting, everywhere.
Ellis MA, Potter BA, Cresawn KO, Weisz OA. Ellis MA, et al. Am J Physiol Renal Physiol. 2006 Oct;291(4):F707-13. doi: 10.1152/ajprenal.00161.2006. Epub 2006 Jun 20. Am J Physiol Renal Physiol. 2006. PMID: 16788143 Review. - N-glycans as apical targeting signals in polarized epithelial cells.
Urquhart P, Pang S, Hooper NM. Urquhart P, et al. Biochem Soc Symp. 2005;(72):39-45. doi: 10.1042/bss0720039. Biochem Soc Symp. 2005. PMID: 15649128 Review.
Cited by
- A size filter at the Golgi regulates apical membrane protein sorting.
de Caestecker C, Macara IG. de Caestecker C, et al. Nat Cell Biol. 2024 Oct;26(10):1678-1690. doi: 10.1038/s41556-024-01500-0. Epub 2024 Sep 5. Nat Cell Biol. 2024. PMID: 39237743 - Regulation of the apico-basolateral trafficking polarity of the homologous copper-ATPases ATP7A and ATP7B.
Ruturaj, Mishra M, Saha S, Maji S, Rodriguez-Boulan E, Schreiner R, Gupta A. Ruturaj, et al. J Cell Sci. 2024 Mar 1;137(5):jcs261258. doi: 10.1242/jcs.261258. Epub 2023 Nov 30. J Cell Sci. 2024. PMID: 38032054 Free PMC article. - Calcium-binding Cab45 regulates the polarized apical secretion of soluble proteins in epithelial cells.
Liu D, Paladino S, Zurzolo C, Lebreton S. Liu D, et al. Mol Biol Cell. 2023 Jul 1;34(8):br12. doi: 10.1091/mbc.E22-12-0549. Epub 2023 May 10. Mol Biol Cell. 2023. PMID: 37163315 Free PMC article. - Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.
Sandoval L, Labarca M, Retamal C, Sánchez P, Larraín J, González A. Sandoval L, et al. Front Cell Dev Biol. 2022 Dec 7;10:833175. doi: 10.3389/fcell.2022.833175. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36568977 Free PMC article. - The Synaptic Extracellular Matrix: Long-Lived, Stable, and Still Remarkably Dynamic.
Dankovich TM, Rizzoli SO. Dankovich TM, et al. Front Synaptic Neurosci. 2022 Mar 8;14:854956. doi: 10.3389/fnsyn.2022.854956. eCollection 2022. Front Synaptic Neurosci. 2022. PMID: 35350469 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources