Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans - PubMed (original) (raw)

. 1995 Nov 9;378(6553):196-9.

doi: 10.1038/378196a0.

Affiliations

• PMID: 7477324
• DOI: 10.1038/378196a0

Defective recycling of synaptic vesicles in synaptotagmin mutants of Caenorhabditis elegans

E M Jorgensen et al. Nature. 1995.

Abstract

Synaptotagmin, an integral membrane protein of the synaptic vesicle, binds calcium and interacts with proteins of the plasma membrane. These observations suggest several possible functions for synaptotagmin in synaptic vesicle dynamics: it could facilitate exocytosis by promoting calcium-dependent fusion, inhibit exocytosis by preventing fusion, or facilitate endocytosis of synaptic vesicles from the plasma membrane by acting as a receptor for the endocytotic proteins of the clathrin AP2 complex. Here we show that synaptic vesicles are depleted at synaptic terminals in synaptotagmin mutants of the nematode Caenorhabditis elegans. This depletion is not caused by a defect in transport or by increased synaptic vesicle release, but rather by a defect in retrieval or synaptic vesicles from the plasma membrane. Thus we propose that, as well as being involved in exocytosis, synaptotagmin functions in vesicular recycling.

PubMed Disclaimer

Similar articles

• AP180 maintains the distribution of synaptic and vesicle proteins in the nerve terminal and indirectly regulates the efficacy of Ca2+-triggered exocytosis.
  Bao H, Daniels RW, MacLeod GT, Charlton MP, Atwood HL, Zhang B. Bao H, et al. J Neurophysiol. 2005 Sep;94(3):1888-903. doi: 10.1152/jn.00080.2005. Epub 2005 May 11. J Neurophysiol. 2005. PMID: 15888532
• Synaptic vesicle fusion and synaptotagmin: 2B or not 2B?
  O'Connor V, Lee AG. O'Connor V, et al. Nat Neurosci. 2002 Sep;5(9):823-4. doi: 10.1038/nn0902-823. Nat Neurosci. 2002. PMID: 12196805 Review. No abstract available.
• Binding of synaptotagmin to the alpha-latrotoxin receptor implicates both in synaptic vesicle exocytosis.
  Petrenko AG, Perin MS, Davletov BA, Ushkaryov YA, Geppert M, Südhof TC. Petrenko AG, et al. Nature. 1991 Sep 5;353(6339):65-8. doi: 10.1038/353065a0. Nature. 1991. PMID: 1881448
• Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo.
  Poskanzer KE, Marek KW, Sweeney ST, Davis GW. Poskanzer KE, et al. Nature. 2003 Dec 4;426(6966):559-63. doi: 10.1038/nature02184. Epub 2003 Nov 23. Nature. 2003. PMID: 14634669
• The synaptic vesicle cycle: a cascade of protein-protein interactions.
  Südhof TC. Südhof TC. Nature. 1995 Jun 22;375(6533):645-53. doi: 10.1038/375645a0. Nature. 1995. PMID: 7791897 Review.

Cited by

• Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1.
  Tsemperouli M, Cheppali SK, Molina FR, Chetrit D, Landajuela A, Toomre D, Karatekin E. Tsemperouli M, et al. bioRxiv [Preprint]. 2024 Sep 12:2024.09.12.612660. doi: 10.1101/2024.09.12.612660. bioRxiv. 2024. PMID: 39314345 Free PMC article. Preprint.
• Toolkits for detailed and high-throughput interrogation of synapses in C. elegans.
  Majeed M, Han H, Zhang K, Cao WX, Liao CP, Hobert O, Lu H. Majeed M, et al. Elife. 2024 Jan 15;12:RP91775. doi: 10.7554/eLife.91775. Elife. 2024. PMID: 38224479 Free PMC article.
• The juxtamembrane linker of synaptotagmin 1 regulates Ca2+ binding via liquid-liquid phase separation.
  Mehta N, Mondal S, Watson ET, Cui Q, Chapman ER. Mehta N, et al. Nat Commun. 2024 Jan 4;15(1):262. doi: 10.1038/s41467-023-44414-5. Nat Commun. 2024. PMID: 38177243 Free PMC article.
• Synaptotagmin-7 outperforms synaptotagmin-1 to promote the formation of large, stable fusion pores via robust membrane penetration.
  Courtney KC, Mandal T, Mehta N, Wu L, Li Y, Das D, Cui Q, Chapman ER. Courtney KC, et al. Nat Commun. 2023 Nov 27;14(1):7761. doi: 10.1038/s41467-023-42497-8. Nat Commun. 2023. PMID: 38012142 Free PMC article.
• Double mutation of open syntaxin and UNC-18 P334A leads to excitatory-inhibitory imbalance and impairs multiple aspects of C. elegans behavior.
  Huang M, Wang Y, Chow CH, Stepien KP, Indrawinata K, Xu J, Argiropoulos P, Xie X, Sugita K, Tien CW, Lee S, Monnier PP, Rizo J, Gao S, Sugita S. Huang M, et al. bioRxiv [Preprint]. 2023 Nov 10:2023.08.18.553709. doi: 10.1101/2023.08.18.553709. bioRxiv. 2023. PMID: 37645974 Free PMC article. Preprint.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program