Prevention of diabetes-associated embryopathy by overexpression of the free radical scavenger copper zinc superoxide dismutase in transgenic mouse embryos - PubMed (original) (raw)
Prevention of diabetes-associated embryopathy by overexpression of the free radical scavenger copper zinc superoxide dismutase in transgenic mouse embryos
Z J Hagay et al. Am J Obstet Gynecol. 1995 Oct.
Abstract
Objectives: It has recently been suggested that oxygen free radicals are involved in the high incidence of fetal dysmorphogenesis that is associated with diabetic pregnancies. The purpose of the current investigation was to study the effect of copper zinc superoxide dismutase, a free radical scavenging enzyme, on the prevention of diabetes-associated embryopathy in mice.
Study design: Mice used in this study were either transgenic, bearing the human copper zinc superoxide dismutase gene, or nontransgenic controls. Diabetes was generated by streptozotocin administration on days 6 and 7 of gestation. Hyperglycemia developed on day 8 and was maintained through day 10 (critical period of organogenesis). On day 10 fetuses were examined for external anomalies, and their crown-rump lengths and deoxyribonucleic acid content were determined.
Results: Induction of maternal diabetes produced a significant reduction in mean crown-rump length of control embryos (4.48 +/- 0.7 mm vs 3.65 +/- 0.6 mm, p = 0.0001), whereas transgenic embryos were not affected (4.72 +/- 0.6 mm vs 4.45 +/- 0.8 mm, p > 0.05). After induction of diabetes fetal loss and malformation rates were significantly higher in control embryos (6.0% vs 23.8% and 8.4% vs 16.5%, respectively). Transgenic embryos were practically unaffected by diabetes and showed fetal loss and malformation rates of 5.9% and 4.4%, respectively, after induction of diabetes.
Conclusions: Elevated levels of copper zinc superoxide dismutase, a key enzyme in the metabolism of free oxygen radicals, elicit a protective effect against diabetes-associated embryopathy.
Similar articles
- Free radical scavenging enzymes in fetal dysmorphogenesis among offspring of diabetic rats.
Sivan E, Lee YC, Wu YK, Reece EA. Sivan E, et al. Teratology. 1997 Dec;56(6):343-9. doi: 10.1002/(SICI)1096-9926(199712)56:6<343::AID-TERA1>3.0.CO;2-X. Teratology. 1997. PMID: 9485543 - Maternal blood glucose levels determine the severity of diabetic embryopathy in mice with different expression of copper-zinc superoxide dismutase (CuZnSOD).
Zabihi S, Wentzel P, Eriksson UJ. Zabihi S, et al. Toxicol Sci. 2008 Sep;105(1):166-72. doi: 10.1093/toxsci/kfn101. Epub 2008 May 22. Toxicol Sci. 2008. PMID: 18502742 - Altered levels of scavenging enzymes in embryos subjected to a diabetic environment.
Forsberg H, Borg LA, Cagliero E, Eriksson UJ. Forsberg H, et al. Free Radic Res. 1996 Jun;24(6):451-9. doi: 10.3109/10715769609088044. Free Radic Res. 1996. PMID: 8804988 - [Recent advances in the pathogenesis of diabetic embryopathy].
García G D, García D R. García G D, et al. Rev Med Chil. 2009 Dec;137(12):1627-35. Epub 2010 Mar 17. Rev Med Chil. 2009. PMID: 20361141 Review. Spanish. - The role of free radicals and membrane lipids in diabetes-induced congenital malformations.
Reece EA, Homko CJ, Wu YK, Wiznitzer A. Reece EA, et al. J Soc Gynecol Investig. 1998 Jul-Aug;5(4):178-87. doi: 10.1016/s1071-5576(98)00008-2. J Soc Gynecol Investig. 1998. PMID: 9699175 Review.
Cited by
- New concepts in diabetic embryopathy.
Zhao Z, Reece EA. Zhao Z, et al. Clin Lab Med. 2013 Jun;33(2):207-33. doi: 10.1016/j.cll.2013.03.017. Epub 2013 Apr 19. Clin Lab Med. 2013. PMID: 23702113 Free PMC article. Review. - Understanding diabetic teratogenesis: where are we now and where are we going?
Zabihi S, Loeken MR. Zabihi S, et al. Birth Defects Res A Clin Mol Teratol. 2010 Oct;88(10):779-90. doi: 10.1002/bdra.20704. Birth Defects Res A Clin Mol Teratol. 2010. PMID: 20706996 Free PMC article. Review. - Modulatory Mechanism of Polyphenols and Nrf2 Signaling Pathway in LPS Challenged Pregnancy Disorders.
Hussain T, Tan B, Liu G, Murtaza G, Rahu N, Saleem M, Yin Y. Hussain T, et al. Oxid Med Cell Longev. 2017;2017:8254289. doi: 10.1155/2017/8254289. Epub 2017 Aug 23. Oxid Med Cell Longev. 2017. PMID: 29138679 Free PMC article. Review. - Pregnancy in Type 1 Diabetes Mellitus: How Special are Special Issues?
Magon N, Chauhan M. Magon N, et al. N Am J Med Sci. 2012 Jun;4(6):250-6. doi: 10.4103/1947-2714.97202. N Am J Med Sci. 2012. PMID: 22754875 Free PMC article. - Advances in revealing the molecular targets downstream of oxidative stress-induced proapoptotic kinase signaling in diabetic embryopathy.
Wang F, Reece EA, Yang P. Wang F, et al. Am J Obstet Gynecol. 2015 Aug;213(2):125-34. doi: 10.1016/j.ajog.2015.01.016. Epub 2015 Jan 13. Am J Obstet Gynecol. 2015. PMID: 25595581 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical