Analysis of melanoma cells stably transfected with beta 1,4GalNAc transferase (GM2/GD2 synthase) cDNA: relative glycosyltransferase levels play a dominant role in determining ganglioside expression - PubMed (original) (raw)

. 1995 Oct 20;323(1):11-8.

doi: 10.1006/abbi.1995.0003.

Affiliations

• PMID: 7487055
• DOI: 10.1006/abbi.1995.0003

Analysis of melanoma cells stably transfected with beta 1,4GalNAc transferase (GM2/GD2 synthase) cDNA: relative glycosyltransferase levels play a dominant role in determining ganglioside expression

S Ruan et al. Arch Biochem Biophys. 1995.

Abstract

Previous studies (Y. Nagata, S. Yamashiro, J. Yodoi, K. O. Lloyd, H. Shiku, and K. Furukawa (1992) J. Biol. Chem. 267, 12082-12089) had isolated a putative cDNA coding for beta 1,4 N-acetylgalactosaminyltransferase (GM2/GD2 synthase) and demonstrated the presence of GM2 and/or GD2 gangliosides in melanoma cell lines stably transfected with this gene. We have now measured the levels of glycosyltransferase activities and mRNA levels in five transfected cell lines in comparison with their parent cell lines (mouse melanoma B16 and human melanoma MeWo). Membrane preparations from the transfected cell lines demonstrated de novo synthesis of GM2 or GD2 in in vitro assays using GM3 or GD3, respectively, as ganglioside acceptors. The enzyme levels, however, varied considerably among the different transfectants, as did the mRNA levels for the beta 1,4 Gal-NAc-transferase. The effect of the transfected gene on levels of preexisting glycosyltransferases involved in ganglioside biosynthesis was also measured and the ganglioside composition of the cell lines was determined. The level of beta 1,4 GalNAc-transferase expressed in the different cell lines was found to dramatically influence the overall ganglioside composition of the cell. In the transfected cell line with the highest levels of the transferase, for example, biosynthesis was almost completely redirected away from the b pathway to the a pathway with the resulting expression of only GM2. These data from this family of related cell lines clearly demonstrate the primary roles that relative glycosyltransferase levels play in determining the ganglioside composition of cells.

PubMed Disclaimer

Similar articles

• Beta1,4-N-acetylgalactosaminyltransferase--GM2/GD2 synthase: a key enzyme to control the synthesis of brain-enriched complex gangliosides.
  Furukawa K, Takamiya K, Furukawa K. Furukawa K, et al. Biochim Biophys Acta. 2002 Dec 19;1573(3):356-62. doi: 10.1016/s0304-4165(02)00403-8. Biochim Biophys Acta. 2002. PMID: 12417418 Review.
• Disordered testosterone transport in mice lacking the ganglioside GM2/GD2 synthase gene.
  Furukawa K, Takamiya K, Ohmi Y, Bhuiyan RH, Tajima O, Furukawa K. Furukawa K, et al. FEBS Open Bio. 2023 Sep;13(9):1615-1624. doi: 10.1002/2211-5463.13603. Epub 2023 Apr 6. FEBS Open Bio. 2023. PMID: 36999634 Free PMC article. Review.

Cited by

• Biosynthesis and functions of gangliosides: recent advances.
  Lloyd KO, Furukawa K. Lloyd KO, et al. Glycoconj J. 1998 Jul;15(7):627-36. doi: 10.1023/a:1006924128550. Glycoconj J. 1998. PMID: 9881769 Review. No abstract available.
• A Compound Heterozygous Pathogenic Variant in B4GALNT1 Is Associated With Axonal Charcot-Marie-Tooth Disease.
  Hong JM, Jeon H, Choi YC, Cho H, Hong YB, Park HJ. Hong JM, et al. J Clin Neurol. 2021 Oct;17(4):534-540. doi: 10.3988/jcn.2021.17.4.534. J Clin Neurol. 2021. PMID: 34595861 Free PMC article.
• Neuronal expression of GalNAc transferase is sufficient to prevent the age-related neurodegenerative phenotype of complex ganglioside-deficient mice.
  Yao D, McGonigal R, Barrie JA, Cappell J, Cunningham ME, Meehan GR, Fewou SN, Edgar JM, Rowan E, Ohmi Y, Furukawa K, Furukawa K, Brophy PJ, Willison HJ. Yao D, et al. J Neurosci. 2014 Jan 15;34(3):880-91. doi: 10.1523/JNEUROSCI.3996-13.2014. J Neurosci. 2014. PMID: 24431446 Free PMC article.
• C1q-targeted inhibition of the classical complement pathway prevents injury in a novel mouse model of acute motor axonal neuropathy.
  McGonigal R, Cunningham ME, Yao D, Barrie JA, Sankaranarayanan S, Fewou SN, Furukawa K, Yednock TA, Willison HJ. McGonigal R, et al. Acta Neuropathol Commun. 2016 Mar 2;4:23. doi: 10.1186/s40478-016-0291-x. Acta Neuropathol Commun. 2016. PMID: 26936605 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • GlyGen glycoinformatics resource