In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency - PubMed (original) (raw)
. 1995 Dec;1(12):1284-90.
doi: 10.1038/nm1295-1284.
Affiliations
- PMID: 7489410
- DOI: 10.1038/nm1295-1284
In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency
T W Chun et al. Nat Med. 1995 Dec.
Abstract
Although it is presumed that the integration of HIV-1 into the genome of infected CD4+ T lymphocytes allows viral persistence, there has been little direct evidence that CD4+ T cells with integrated provirus function as a latent reservoir for HIV-1 in infected individuals. Using resting CD4+ T-cell populations of extremely high purity and a novel assay that selectively and unambiguously detects integrated HIV-1, we show that resting CD4+ T cells harbouring integrated provirus are present in some infected individuals. However, these cells do not accumulate within the circulating pool of resting CD4+ T cells in the early stages of HIV-1 infection and do not accumulate even after prolonged periods in long-term survivors of HIV-1 infection. These results suggest that because of viral cytopathic effects and/or host effector mechanisms, productively infected CD4+ T cells do not generally survive for long enough to revert to a resting memory state in vivo.
Similar articles
- Analysis of human immunodeficiency virus type 1 gene expression in latently infected resting CD4+ T lymphocytes in vivo.
Hermankova M, Siliciano JD, Zhou Y, Monie D, Chadwick K, Margolick JB, Quinn TC, Siliciano RF. Hermankova M, et al. J Virol. 2003 Jul;77(13):7383-92. doi: 10.1128/jvi.77.13.7383-7392.2003. J Virol. 2003. PMID: 12805437 Free PMC article. - The role of integration and clonal expansion in HIV infection: live long and prosper.
Anderson EM, Maldarelli F. Anderson EM, et al. Retrovirology. 2018 Oct 23;15(1):71. doi: 10.1186/s12977-018-0448-8. Retrovirology. 2018. PMID: 30352600 Free PMC article. Review. - CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses.
Descours B, Petitjean G, López-Zaragoza JL, Bruel T, Raffel R, Psomas C, Reynes J, Lacabaratz C, Levy Y, Schwartz O, Lelievre JD, Benkirane M. Descours B, et al. Nature. 2017 Mar 23;543(7646):564-567. doi: 10.1038/nature21710. Epub 2017 Mar 15. Nature. 2017. PMID: 28297712 - Experimental approaches to the study of HIV-1 latency.
Han Y, Wind-Rotolo M, Yang HC, Siliciano JD, Siliciano RF. Han Y, et al. Nat Rev Microbiol. 2007 Feb;5(2):95-106. doi: 10.1038/nrmicro1580. Nat Rev Microbiol. 2007. PMID: 17224919 Review. - Measuring integrated HIV DNA ex vivo and in vitro provides insights about how reservoirs are formed and maintained.
Pinzone MR, O'Doherty U. Pinzone MR, et al. Retrovirology. 2018 Feb 17;15(1):22. doi: 10.1186/s12977-018-0396-3. Retrovirology. 2018. PMID: 29452580 Free PMC article. Review.
Cited by
- Spatial technologies to evaluate the HIV-1 reservoir and its microenvironment in the lymph node.
Zaman F, Smith ML, Balagopal A, Durand CM, Redd AD, Tobian AAR. Zaman F, et al. mBio. 2024 Aug 14;15(8):e0190924. doi: 10.1128/mbio.01909-24. Epub 2024 Jul 26. mBio. 2024. PMID: 39058091 Free PMC article. Review. - mRNA-based HIV-1 vaccines.
Ahmed S, Herschhorn A. Ahmed S, et al. Clin Microbiol Rev. 2024 Sep 12;37(3):e0004124. doi: 10.1128/cmr.00041-24. Epub 2024 Jul 17. Clin Microbiol Rev. 2024. PMID: 39016564 Review. - Advancing Toward a Human Immunodeficiency Virus Cure: Initial Progress on a Difficult Path.
Margolis DM. Margolis DM. Infect Dis Clin North Am. 2024 Sep;38(3):487-497. doi: 10.1016/j.idc.2024.06.001. Epub 2024 Jul 4. Infect Dis Clin North Am. 2024. PMID: 38969530 Review. - Defining the Effects of PKC Modulator HIV Latency-Reversing Agents on Natural Killer Cells.
Dimapasoc M, Moran JA, Cole SW, Ranjan A, Hourani R, Kim JT, Wender PA, Marsden MD, Zack JA. Dimapasoc M, et al. Pathog Immun. 2024 Apr 24;9(1):108-137. doi: 10.20411/pai.v9i1.673. eCollection 2024. Pathog Immun. 2024. PMID: 38765786 Free PMC article. - Factors associated with unquantifiable total HIV-1 DNA in peripheral blood in persons living with HIV: An observational study.
Ram A, Rascon Velasco V, Mchantaf G, Avettand-Fénoël V, Viard JP. Ram A, et al. J Virus Erad. 2024 Mar 28;10(1):100370. doi: 10.1016/j.jve.2024.100370. eCollection 2024 Mar. J Virus Erad. 2024. PMID: 38596322 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials