Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection - PubMed (original) (raw)

Comparative Study

Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection

Y Huang et al. J Virol. 1995 Dec.

Abstract

We have previously shown that there were no gross deletions or obvious sequence abnormalities within nef of human immunodeficiency virus type 1 (HIV-1) in the 10 long-term survivors studied (Y. Huang, L. Zhang, and D. D. Ho, J. Virol. 69:93-100, 1995). Here we extend our study to examine these nef alleles in a functional context. Using a new technique, termed site-directed gene replacement, we have precisely replaced the nef of an infectious molecular clone, HIV-1HXB2, with nef alleles derived from 10 long-term survivors as well as from a patient with AIDS. The replication properties of these chimeric viruses demonstrated that the nef alleles derived from long-term survivors neither significantly increased nor decreased viral replication, compared with the nef allele of Nef+ HIV-1HXB2 and that derived from a patient with AIDS. However, Nef+ viruses always replicated faster than virus lacking nef. Moreover, single-cell infection analysis by the MAGI assay showed that these chimeric viruses, as well as Nef+ HIV-1HXB2, were more infectious than Nef- HIV-1HXB2 was. Therefore, we conclude that the genotypic and phenotypic features of nef are not likely to account for the nonprogression of HIV-1 infection in the 10 cases studied, unless the function of the nef gene in vivo is not accurately reflected by the in vitro assays we used.

PubMed Disclaimer

Cited by

Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses.
McNamara RP, Costantini LM, Myers TA, Schouest B, Maness NJ, Griffith JD, Damania BA, MacLean AG, Dittmer DP. McNamara RP, et al. mBio. 2018 Feb 6;9(1):e02344-17. doi: 10.1128/mBio.02344-17. mBio. 2018. PMID: 29437924 Free PMC article.
Meta-analysis to test the association of HIV-1 nef amino acid differences and deletions with disease progression.
Pushker R, Jacqué JM, Shields DC. Pushker R, et al. J Virol. 2010 Apr;84(7):3644-53. doi: 10.1128/JVI.01959-09. Epub 2010 Jan 13. J Virol. 2010. PMID: 20071583 Free PMC article.
Elite suppressor-derived HIV-1 envelope glycoproteins exhibit reduced entry efficiency and kinetics.
Lassen KG, Lobritz MA, Bailey JR, Johnston S, Nguyen S, Lee B, Chou T, Siliciano RF, Markowitz M, Arts EJ. Lassen KG, et al. PLoS Pathog. 2009 Apr;5(4):e1000377. doi: 10.1371/journal.ppat.1000377. Epub 2009 Apr 10. PLoS Pathog. 2009. PMID: 19360131 Free PMC article.
Evidence of CD8+ T-cell-mediated selective pressure on human immunodeficiency virus type 1 nef in HLA-B*57+ elite suppressors.
Bailey JR, Brennan TP, O'Connell KA, Siliciano RF, Blankson JN. Bailey JR, et al. J Virol. 2009 Jan;83(1):88-97. doi: 10.1128/JVI.01958-08. Epub 2008 Oct 22. J Virol. 2009. PMID: 18945771 Free PMC article.
Genetic characterization of human immunodeficiency virus type 1 in elite controllers: lack of gross genetic defects or common amino acid changes.
Miura T, Brockman MA, Brumme CJ, Brumme ZL, Carlson JM, Pereyra F, Trocha A, Addo MM, Block BL, Rothchild AC, Baker BM, Flynn T, Schneidewind A, Li B, Wang YE, Heckerman D, Allen TM, Walker BD. Miura T, et al. J Virol. 2008 Sep;82(17):8422-30. doi: 10.1128/JVI.00535-08. Epub 2008 Jun 18. J Virol. 2008. PMID: 18562530 Free PMC article.

References

1. J Virol. 1994 May;68(5):2906-14 - PubMed
1. Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1104-8 - PubMed
1. J Virol. 1995 Jan;69(1):93-100 - PubMed
1. N Engl J Med. 1995 Jan 26;332(4):201-8 - PubMed
1. N Engl J Med. 1995 Jan 26;332(4):228-32 - PubMed

Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection - PubMed (original) (raw)