Cdc42 and PAK-mediated signaling leads to Jun kinase and p38 mitogen-activated protein kinase activation - PubMed (original) (raw)

Comparative Study

. 1995 Nov 24;270(47):27995-8.

doi: 10.1074/jbc.270.47.27995.

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Comparative Study

Cdc42 and PAK-mediated signaling leads to Jun kinase and p38 mitogen-activated protein kinase activation

S Bagrodia et al. J Biol Chem. 1995.

Free article

Abstract

The PAK family of protein kinases has been suggested as a potential target of the Cdc42 and Rac GTPases based on studies in vitro. We show that PAK-3 is activated by Cdc42 in vivo. Both, activated (GTPase-defective) Cdc42 and a constitutively active PAK-3 mutant stimulated the activity of Jun kinase 1 (JNK1) in transfected cells. Activated Cdc42 also stimulated the activity of the related p38 mitogen-activated protein kinase but was a less effective activator of ERK2. The effect of Cdc42 on JNK activity was similar to that of the potent inflammatory cytokine interleukin-1 (IL-1). The observation that a dominant-negative Cdc42 mutant inhibited IL-1 activation of JNK1 indicates a role for Cdc42 in IL-1 signaling. These results suggest that Cdc42 and PAK may mediate the effects of cytokines on transcriptional regulation.

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