Induction of apoptotic cell death in mouse lymphoma and human leukemia cell lines by a calcium-binding protein complex, calprotectin, derived from inflammatory peritoneal exudate cells - PubMed (original) (raw)
Induction of apoptotic cell death in mouse lymphoma and human leukemia cell lines by a calcium-binding protein complex, calprotectin, derived from inflammatory peritoneal exudate cells
S Yui et al. J Leukoc Biol. 1995 Dec.
Abstract
We have previously shown that the calcium-binding protein complex, calprotectin, purified from rat inflammatory peritoneal cells exerts marked cytotoxic activity against rat, mouse, and human tumor cells. We studied here whether the cytotoxicity is caused by induction of apoptosis, using mouse EL-4 lymphoma and human MOLT-4 leukemia lines as targets. The rat calprotectin sample inhibited [3H]thymidine incorporation into these cells by partially 24 h and almost completely in 48 h of culture at concentrations of 100-200 micrograms/ml. Morphological changes, that is, loss of cell volume and nuclear condensation and/or fragmentation, appeared in both cell types cultured with calprotectin from 20 h, and such apoptotic cells subsequently increased in number to compose the great majority of the cells at 40 h. Cell death, measured by stainability with trypan blue, lagged behind the emergence of the apoptotic morphology by about 2 and 10 h in EL-4 and MOLT-4 cells, respectively. DNA fragmentation was observed in EL-4 cells cultured with calprotectin, whereas it was not observed in MOLT-4 cells, consistent with results of flow cytometry showing that loss of cell DNA content caused by the factor was greater in EL-4 cells. The data indicate that calprotectin induces the apoptosis of certain tumor cells but that the occurrence of DNA fragmentation is dependent on cell type. Finally, the apoptosis-inducing activity of the calprotectin sample was abrogated by the presence of 10 microM zinc, whereas it was not affected by 5 mM calcium or magnesium.
Similar articles
- Calprotectin (S100A8/S100A9), an inflammatory protein complex from neutrophils with a broad apoptosis-inducing activity.
Yui S, Nakatani Y, Mikami M. Yui S, et al. Biol Pharm Bull. 2003 Jun;26(6):753-60. doi: 10.1248/bpb.26.753. Biol Pharm Bull. 2003. PMID: 12808281 Review. - The leucocyte protein L1 (calprotectin): a putative nonspecific defence factor at epithelial surfaces.
Brandtzaeg P, Gabrielsen TO, Dale I, Müller F, Steinbakk M, Fagerhol MK. Brandtzaeg P, et al. Adv Exp Med Biol. 1995;371A:201-6. doi: 10.1007/978-1-4615-1941-6_41. Adv Exp Med Biol. 1995. PMID: 8525906 Review.
Cited by
- Narciclasine improves outcome in sepsis among neonatal rats via inhibition of calprotectin and alleviating inflammatory responses.
Kingsley MK, Bhat BV, Badhe BA, Dhas BB, Parija SC. Kingsley MK, et al. Sci Rep. 2020 Feb 19;10(1):2947. doi: 10.1038/s41598-020-59716-7. Sci Rep. 2020. PMID: 32076015 Free PMC article. - S100A8/A9 in Inflammation.
Wang S, Song R, Wang Z, Jing Z, Wang S, Ma J. Wang S, et al. Front Immunol. 2018 Jun 11;9:1298. doi: 10.3389/fimmu.2018.01298. eCollection 2018. Front Immunol. 2018. PMID: 29942307 Free PMC article. Review. - Alarmins and immunity.
Yang D, Han Z, Oppenheim JJ. Yang D, et al. Immunol Rev. 2017 Nov;280(1):41-56. doi: 10.1111/imr.12577. Immunol Rev. 2017. PMID: 29027222 Free PMC article. Review. - Calprotectin as a diagnostic tool for inflammatory bowel diseases.
Chatzikonstantinou M, Konstantopoulos P, Stergiopoulos S, Kontzoglou K, Verikokos C, Perrea D, Dimitroulis D. Chatzikonstantinou M, et al. Biomed Rep. 2016 Oct;5(4):403-407. doi: 10.3892/br.2016.751. Epub 2016 Sep 7. Biomed Rep. 2016. PMID: 27699005 Free PMC article. - High expression of S100A8 gene is associated with drug resistance to etoposide and poor prognosis in acute myeloid leukemia through influencing the apoptosis pathway.
Yang XY, Zhang MY, Zhou Q, Wu SY, Zhao Y, Gu WY, Pan J, Cen JN, Chen ZX, Guo WG, Chen CS, Yan WH, Hu SY. Yang XY, et al. Onco Targets Ther. 2016 Aug 8;9:4887-99. doi: 10.2147/OTT.S101594. eCollection 2016. Onco Targets Ther. 2016. PMID: 27540302 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources