TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a multisubunit complex that appears to recognize G/UAG repeats in the trpEDCFBA and trpG transcripts - PubMed (original) (raw)
Comparative Study
. 1994 Jun 17;269(24):16597-604.
Affiliations
- PMID: 7515880
Free article
Comparative Study
TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a multisubunit complex that appears to recognize G/UAG repeats in the trpEDCFBA and trpG transcripts
P Babitzke et al. J Biol Chem. 1994.
Free article
Abstract
A filter binding assay was developed to study interactions between purified TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, and trp specific transcripts. TRAP formed stable complexes with trpEDCFBA leader RNA; binding was L-tryptophan-dependent and was complete within 60 s. TRAP binds to a segment of the trp leader transcript that includes part of an RNA antiterminator structure. Binding to this segment allows formation of an RNA terminator structure, thereby promoting transcription termination. Using several trpEDCFBA leader deletion transcripts, we identified several closely spaced trinucleotide repeats (seven GAG and four UAG repeats) in the trp leader transcript that appeared to be required for TRAP binding. We also showed that TRAP binds to a segment of the trpG transcript that includes the trpG ribosome binding site; the nucleotide sequence of this segment contains several appropriately spaced trinucleotide repeats (seven GAG, one UAG, and one AAG). TRAP binding to the trpG transcript would block translation initiation. RNA footprint analysis confirmed interaction between TRAP and the trinucleotide repeats in the various transcripts. TRAP, in the presence or absence of L-tryptophan, appears to consist of 11 or 12 identical 8-kDa subunits. Our findings suggest that each tryptophan-activated TRAP subunit can bind one G/UAG repeat in a target transcript. Multiple protein-RNA interactions are required for stable association.
Similar articles
- trp RNA-binding attenuation protein-5' stem-loop RNA interaction is required for proper transcription attenuation control of the Bacillus subtilis trpEDCFBA operon.
Du H, Yakhnin AV, Dharmaraj S, Babitzke P. Du H, et al. J Bacteriol. 2000 Apr;182(7):1819-27. doi: 10.1128/JB.182.7.1819-1827.2000. J Bacteriol. 2000. PMID: 10714985 Free PMC article. - The trp RNA-binding attenuation protein regulates TrpG synthesis by binding to the trpG ribosome binding site of Bacillus subtilis.
Du H, Tarpey R, Babitzke P. Du H, et al. J Bacteriol. 1997 Apr;179(8):2582-6. doi: 10.1128/jb.179.8.2582-2586.1997. J Bacteriol. 1997. PMID: 9098056 Free PMC article. - Regulation of tryptophan biosynthesis: Trp-ing the TRAP or how Bacillus subtilis reinvented the wheel.
Babitzke P. Babitzke P. Mol Microbiol. 1997 Oct;26(1):1-9. doi: 10.1046/j.1365-2958.1997.5541915.x. Mol Microbiol. 1997. PMID: 9383185 Review.
Cited by
- The genetic code as expressed through relationships between mRNA structure and protein function.
Mauger DM, Siegfried NA, Weeks KM. Mauger DM, et al. FEBS Lett. 2013 Apr 17;587(8):1180-1188. doi: 10.1016/j.febslet.2013.03.002. Epub 2013 Mar 13. FEBS Lett. 2013. PMID: 23499436 Free PMC article. Review. - Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR.
Nie M, Htun H. Nie M, et al. Nucleic Acids Res. 2006;34(19):5528-40. doi: 10.1093/nar/gkl584. Epub 2006 Oct 5. Nucleic Acids Res. 2006. PMID: 17023487 Free PMC article. - Mechanism of NusG-stimulated pausing, hairpin-dependent pause site selection and intrinsic termination at overlapping pause and termination sites in the Bacillus subtilis trp leader.
Yakhnin AV, Babitzke P. Yakhnin AV, et al. Mol Microbiol. 2010 May;76(3):690-705. doi: 10.1111/j.1365-2958.2010.07126.x. Epub 2010 Apr 8. Mol Microbiol. 2010. PMID: 20384694 Free PMC article. - A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Sarsero JP, Merino E, Yanofsky C. Sarsero JP, et al. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2656-61. doi: 10.1073/pnas.050578997. Proc Natl Acad Sci U S A. 2000. PMID: 10706627 Free PMC article. - trp RNA-binding attenuation protein-5' stem-loop RNA interaction is required for proper transcription attenuation control of the Bacillus subtilis trpEDCFBA operon.
Du H, Yakhnin AV, Dharmaraj S, Babitzke P. Du H, et al. J Bacteriol. 2000 Apr;182(7):1819-27. doi: 10.1128/JB.182.7.1819-1827.2000. J Bacteriol. 2000. PMID: 10714985 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases