Nodavirus RNA replication: mechanism and harnessing to vaccinia virus recombinants - PubMed (original) (raw)
Nodavirus RNA replication: mechanism and harnessing to vaccinia virus recombinants
L A Ball et al. Arch Virol Suppl. 1994.
Abstract
In order to harness RNA replication for the amplification of mRNAs expressed from recombinant vectors and vaccines, we constructed a VV recombinant that expressed the RNA replicase encoded in the larger genomic segment of the nodavirus FHV. When both termini of the VV-derived transcript were correct, the encoded enzyme replicated its own mRNA, and replication dominated the RNA synthetic capacity of the cell. The smaller genomic segment of FHV could also be replicated by the enzyme when supplied in trans, either by coinfection with another VV recombinant or by transfection of an appropriate plasmid. However, two requirements had to be fulfilled for replication of the smaller FHV RNA segment. The first was the prior replication of the larger genomic segment, which was interpreted as a mechanism to achieve sufficient replicase synthesis before the onset of coat protein synthesis. The second was the presence in the smaller genomic RNA of an internal region between about nucleotides 525-620. Work is in progress to elucidate the reasons for these requirements for RNA 2 replication.
Similar articles
- Cellular expression of a functional nodavirus RNA replicon from vaccinia virus vectors.
Ball LA. Ball LA. J Virol. 1992 Apr;66(4):2335-45. doi: 10.1128/JVI.66.4.2335-2345.1992. J Virol. 1992. PMID: 1548766 Free PMC article. - Replication of the genomic RNA of a positive-strand RNA animal virus from negative-sense transcripts.
Ball LA. Ball LA. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12443-7. doi: 10.1073/pnas.91.26.12443. Proc Natl Acad Sci U S A. 1994. PMID: 7809056 Free PMC article. - cis-acting requirements for the replication of flock house virus RNA 2.
Ball LA, Li Y. Ball LA, et al. J Virol. 1993 Jun;67(6):3544-51. doi: 10.1128/JVI.67.6.3544-3551.1993. J Virol. 1993. PMID: 8497063 Free PMC article. - Protein-protein interactions and glycerophospholipids in bromovirus and nodavirus RNA replication.
Ahlquist P, Wu SX, Kaesberg P, Kao CC, Quadt R, DeJong W, Hershberger R. Ahlquist P, et al. Arch Virol Suppl. 1994;9:135-45. doi: 10.1007/978-3-7091-9326-6_14. Arch Virol Suppl. 1994. PMID: 8032245 Review. - Replication strategies of the single stranded RNA viruses of eukaryotes.
Strauss EG, Strauss JH. Strauss EG, et al. Curr Top Microbiol Immunol. 1983;105:1-98. doi: 10.1007/978-3-642-69159-1_1. Curr Top Microbiol Immunol. 1983. PMID: 6354610 Review. No abstract available.
Cited by
- Latent infection of a new alphanodavirus in an insect cell line.
Li TC, Scotti PD, Miyamura T, Takeda N. Li TC, et al. J Virol. 2007 Oct;81(20):10890-6. doi: 10.1128/JVI.00807-07. Epub 2007 Aug 8. J Virol. 2007. PMID: 17686877 Free PMC article. - Replication of flock house virus RNAs from primary transcripts made in cells by RNA polymerase II.
Johnson KL, Ball LA. Johnson KL, et al. J Virol. 1997 Apr;71(4):3323-7. doi: 10.1128/JVI.71.4.3323-3327.1997. J Virol. 1997. PMID: 9060703 Free PMC article. - Fecal virome analysis of three carnivores reveals a novel nodavirus and multiple gemycircularviruses.
Conceição-Neto N, Zeller M, Heylen E, Lefrère H, Mesquita JR, Matthijnssens J. Conceição-Neto N, et al. Virol J. 2015 May 20;12:79. doi: 10.1186/s12985-015-0305-5. Virol J. 2015. PMID: 25986582 Free PMC article. - Transgenerational inheritance of an acquired small RNA-based antiviral response in C. elegans.
Rechavi O, Minevich G, Hobert O. Rechavi O, et al. Cell. 2011 Dec 9;147(6):1248-56. doi: 10.1016/j.cell.2011.10.042. Epub 2011 Nov 23. Cell. 2011. PMID: 22119442 Free PMC article. - Isolation and characterization of a novel alphanodavirus.
Bai H, Wang Y, Li X, Mao H, Li Y, Han S, Shi Z, Chen X. Bai H, et al. Virol J. 2011 Jun 19;8:311. doi: 10.1186/1743-422X-8-311. Virol J. 2011. PMID: 21682922 Free PMC article.