Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways - PubMed (original) (raw)
. 1994 Aug 25;370(6491):650-2.
doi: 10.1038/370650a0.
Affiliations
- PMID: 7520535
- DOI: 10.1038/370650a0
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways
B Lowin et al. Nature. 1994.
Abstract
The recent generation of perforin knock-out mice has demonstrated a crucial role for the pore-forming perforin in cytolytic T-lymphocyte (CTL)-mediated cytolysis. Perforin-deficient mice failed to clear lymphocytic choriomeningitis virus in vivo, yet substantial killing activity still remained in perforin-free CTLs in vitro, indicating the presence of (a) further lytic pathway(s). Fas is an apoptosis-signalling receptor molecule on the surface of a number of different cells. Here we report that both perforin-deficient and Fas-ligand-deficient CTLs show impaired lytic activity on all target cells tested. The killing activity was completely abolished when both pathways were inactivated by using target cells from Fas-receptor-deficient lpr mice and perforin-free CTL effector cells. Fas-ligand-based killing activity was triggered upon T-cell receptor occupancy and was directed to the cognate target cell. Thus, two complementary, specific cytotoxic mechanisms are functional in CTLs, one based on the secretion of lytic proteins and one which depends on cell-surface ligand-receptor interaction.
Similar articles
- Concanamycin A, a powerful tool for characterization and estimation of contribution of perforin- and Fas-based lytic pathways in cell-mediated cytotoxicity.
Kataoka T, Shinohara N, Takayama H, Takaku K, Kondo S, Yonehara S, Nagai K. Kataoka T, et al. J Immunol. 1996 May 15;156(10):3678-86. J Immunol. 1996. PMID: 8621902 - MRL/lpr CD4- CD8- and CD8+ T cells, respectively, mediate Fas-dependent and perforin cytotoxic pathways.
Benihoud K, Bonardelle D, Bobé P, Kiger N. Benihoud K, et al. Eur J Immunol. 1997 Feb;27(2):415-20. doi: 10.1002/eji.1830270211. Eur J Immunol. 1997. PMID: 9045912 - Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis.
Bachmann MF, Ohteki T, Faienza KM, Zakarian A, Kägi D, Speiser DE, Ohashi PS. Bachmann MF, et al. J Immunol. 1997 Nov 1;159(9):4165-70. J Immunol. 1997. PMID: 9379009 - Involvement of granule proteins in T-cell-mediated cytolysis.
Krähenbühl O, Tschopp J. Krähenbühl O, et al. Nat Immun Cell Growth Regul. 1990;9(4):274-82. Nat Immun Cell Growth Regul. 1990. PMID: 2215515 Review. - The CTL's kiss of death.
Berke G. Berke G. Cell. 1995 Apr 7;81(1):9-12. doi: 10.1016/0092-8674(95)90365-8. Cell. 1995. PMID: 7536631 Review.
Cited by
- Murine Models of Familial Cytokine Storm Syndromes.
Volkmer B, Marchetti T, Aichele P, Schmid JP. Volkmer B, et al. Adv Exp Med Biol. 2024;1448:481-496. doi: 10.1007/978-3-031-59815-9_33. Adv Exp Med Biol. 2024. PMID: 39117835 Review. - Exploring GZMK as a prognostic marker and predictor of immunotherapy response in breast cancer: unveiling novel insights into treatment outcomes.
Li Z, Xie Q, Zhao F, Huo X, Ren D, Liu Z, Zhou X, Shen G, Zhao J. Li Z, et al. J Cancer Res Clin Oncol. 2024 Jun 4;150(6):286. doi: 10.1007/s00432-024-05791-6. J Cancer Res Clin Oncol. 2024. PMID: 38833021 Free PMC article. - Immune modulation in malignant pleural effusion: from microenvironment to therapeutic implications.
Ge S, Zhao Y, Liang J, He Z, Li K, Zhang G, Hua B, Zheng H, Guo Q, Qi R, Shi Z. Ge S, et al. Cancer Cell Int. 2024 Mar 12;24(1):105. doi: 10.1186/s12935-024-03211-w. Cancer Cell Int. 2024. PMID: 38475858 Free PMC article. Review. - Oxygen-Generating Cryogels Restore T Cell Mediated Cytotoxicity in Hypoxic Tumors.
Colombani T, Eggermont LJ, Hatfield SM, Rogers ZJ, Rezaeeyazdi M, Memic A, Sitkovsky MV, Bencherif SA. Colombani T, et al. Adv Funct Mater. 2021 Sep 9;31(37):2102234. doi: 10.1002/adfm.202102234. Epub 2021 Jun 23. Adv Funct Mater. 2021. PMID: 37745940 Free PMC article. - Critical role of TLR activation in viral replication, persistence, and pathogenicity of Theiler's virus.
Kim BS. Kim BS. Front Immunol. 2023 Apr 20;14:1167972. doi: 10.3389/fimmu.2023.1167972. eCollection 2023. Front Immunol. 2023. PMID: 37153539 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous