Control of transcription processivity in phage lambda: Nus factors strengthen the termination-resistant state of RNA polymerase induced by N antiterminator - PubMed (original) (raw)
Control of transcription processivity in phage lambda: Nus factors strengthen the termination-resistant state of RNA polymerase induced by N antiterminator
J DeVito et al. Proc Natl Acad Sci U S A. 1994.
Abstract
During transcription of phage lambda early operons, the N gene product alters host RNA polymerase (RNAP) so that transcription proceeds through multiple stop signals. Here, we reproduce the essence of N activity with purified components in synthetic transcription units that contain lambda pL promoter and the N-recognition site, nutL, followed by a variety of intrinsic terminators. We show that three host factors (NusA, NusE, and NusG) are essential for N to allow appreciable transcription through multiple terminators and that this persistent antitermination is stimulated by a fourth factor, NusB. Remarkably, in the absence of all four factors, N suppresses various terminators placed near the nut site. This basal antitermination activity of N is enhanced by NusA and is diminished by high salt and temperature. We postulate that N interacts with RNAP directly, inducing the termination-resistant state. While NusA facilitates this interaction, the other factors strengthen it sufficiently over time and distance so that RNAP bypasses multiple terminators. The dispensability of NusB for persistent antitermination in vitro, but not in vivo, raises the possibility that NusB performs two functions: it increases the stability of N antitermination complex and also counteracts an inhibitory factor in the cell.
Similar articles
- Functional importance of regions in Escherichia coli elongation factor NusA that interact with RNA polymerase, the bacteriophage lambda N protein and RNA.
Mah TF, Li J, Davidson AR, Greenblatt J. Mah TF, et al. Mol Microbiol. 1999 Nov;34(3):523-37. doi: 10.1046/j.1365-2958.1999.01618.x. Mol Microbiol. 1999. PMID: 10564494 - Bipartite function of a small RNA hairpin in transcription antitermination in bacteriophage lambda.
Chattopadhyay S, Garcia-Mena J, DeVito J, Wolska K, Das A. Chattopadhyay S, et al. Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):4061-5. doi: 10.1073/pnas.92.9.4061. Proc Natl Acad Sci U S A. 1995. PMID: 7732031 Free PMC article. - Transcription antitermination: the lambda paradigm updated.
Friedman DI, Court DL. Friedman DI, et al. Mol Microbiol. 1995 Oct;18(2):191-200. doi: 10.1111/j.1365-2958.1995.mmi_18020191.x. Mol Microbiol. 1995. PMID: 8709839 Review. - Factor-stimulated intrinsic termination: getting by with a little help from some friends.
Mandell ZF, Zemba D, Babitzke P. Mandell ZF, et al. Transcription. 2022 Aug-Oct;13(4-5):96-108. doi: 10.1080/21541264.2022.2127602. Epub 2022 Sep 25. Transcription. 2022. PMID: 36154805 Free PMC article. Review.
Cited by
- Stochastic kinetic analysis of developmental pathway bifurcation in phage lambda-infected Escherichia coli cells.
Arkin A, Ross J, McAdams HH. Arkin A, et al. Genetics. 1998 Aug;149(4):1633-48. doi: 10.1093/genetics/149.4.1633. Genetics. 1998. PMID: 9691025 Free PMC article. - The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control.
Zengel JM, Jerauld A, Walker A, Wahl MC, Lindahl L. Zengel JM, et al. RNA. 2003 Oct;9(10):1188-97. doi: 10.1261/rna.5400703. RNA. 2003. PMID: 13130133 Free PMC article. - The structure of the transcriptional antiterminator NusB from Escherichia coli.
Altieri AS, Mazzulla MJ, Horita DA, Coats RH, Wingfield PT, Das A, Court DL, Byrd RA. Altieri AS, et al. Nat Struct Biol. 2000 Jun;7(6):470-4. doi: 10.1038/75869. Nat Struct Biol. 2000. PMID: 10881193 Free PMC article. - Control of rRNA transcription in Escherichia coli.
Condon C, Squires C, Squires CL. Condon C, et al. Microbiol Rev. 1995 Dec;59(4):623-45. doi: 10.1128/mr.59.4.623-645.1995. Microbiol Rev. 1995. PMID: 8531889 Free PMC article. Review. - HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.
Tawk CS, Ghattas IR, Smith CA. Tawk CS, et al. J Bacteriol. 2015 Nov;197(22):3573-82. doi: 10.1128/JB.00466-15. Epub 2015 Sep 8. J Bacteriol. 2015. PMID: 26350130 Free PMC article.
References
- Biochemistry. 1987 Apr 21;26(8):2095-101 - PubMed
- Cell. 1981 May;24(2):421-8 - PubMed
- Cell. 1987 Nov 20;51(4):631-41 - PubMed
- Gene. 1987;61(2):135-44 - PubMed
- Proc Natl Acad Sci U S A. 1988 Apr;85(8):2494-8 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources