P-selectin induces the expression of tissue factor on monocytes - PubMed (original) (raw)

P-selectin induces the expression of tissue factor on monocytes

A Celi et al. Proc Natl Acad Sci U S A. 1994.

Abstract

P-selectin on activated platelets and stimulated endothelial cells mediates cell adhesion with monocytes and neutrophils. Since activated platelets induce tissue factor on mononuclear leukocytes, we examined the effect of P-selectin on the expression of tissue factor activity in monocytes. Purified P-selectin stimulated tissue factor expression on mononuclear leukocytes in a dose-dependent manner. Chinese hamster ovary (CHO) cells expressing P-selectin stimulated tissue factor procoagulant activity in purified monocytes, whereas untransfected CHO cells and CHO cells expressing E-selectin did not. Anti-P-selectin antibodies inhibited the effects of purified P-selectin and CHO cells expressing P-selectin on monocytes. Incubation of CHO cells expressing P-selectin with monocytes leads to the development of tissue factor mRNA in monocytes and to the expression of tissue factor antigen on the monocyte surface. These results indicate that P-selectin upregulates the expression of tissue factor on monocytes as well as mediates the binding of platelets and endothelial cells with monocytes and neutrophils. The binding of P-selectin to monocytes in the area of vascular injury may be a component of a mechanism that initiates thrombosis.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nature. 1992 Oct 29;359(6398):848-51 - PubMed
    1. Circulation. 1993 Feb;87(2):454-63 - PubMed
    1. Blood. 1993 Feb 1;81(3):734-44 - PubMed
    1. J Cell Biol. 1993 Apr;121(2):449-59 - PubMed
    1. J Biol Chem. 1993 May 5;268(13):9753-61 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources