Recruitment of mitochondrial cyclophilin to the mitochondrial inner membrane under conditions of oxidative stress that enhance the opening of a calcium-sensitive non-specific channel - PubMed (original) (raw)
Recruitment of mitochondrial cyclophilin to the mitochondrial inner membrane under conditions of oxidative stress that enhance the opening of a calcium-sensitive non-specific channel
C P Connern et al. Biochem J. 1994.
Abstract
Binding of mitochondrial matrix cyclophilin (CyP) to the rat liver mitochondrial membranes was detected by SDS/PAGE and Western blotting with suitable antipeptide antibodies. Binding was not affected by prior exposure of mitochondria to Ca2+, adenine nucleotides or inhibitors of the adenine nucleotide translocase, but was greatly increased by t-butyl hydroperoxide (tBH), phenylarsine oxide or diamide. These all sensitized the opening of the non-specific mitochondrial pore to [Ca2+], and the effect of tBH was shown to be maintained after washing away the tBH, consistent with it being caused by the enhanced CyP binding. The bound CyP did not demonstrate peptidyl-prolyl cis-trans isomerase activity. CyP-binding was prevented by 5 microM cyclosporin A, but not reversed by cyclosporin treatment of the membranes. The effect of tBH on binding was concentration-dependent and maximal within 30 s.
Similar articles
- Recent progress on regulation of the mitochondrial permeability transition pore; a cyclosporin-sensitive pore in the inner mitochondrial membrane.
Bernardi P, Broekemeier KM, Pfeiffer DR. Bernardi P, et al. J Bioenerg Biomembr. 1994 Oct;26(5):509-17. doi: 10.1007/BF00762735. J Bioenerg Biomembr. 1994. PMID: 7896766 Review. - Cyclosporin A binding to mitochondrial cyclophilin inhibits the permeability transition pore and protects hearts from ischaemia/reperfusion injury.
Halestrap AP, Connern CP, Griffiths EJ, Kerr PM. Halestrap AP, et al. Mol Cell Biochem. 1997 Sep;174(1-2):167-72. Mol Cell Biochem. 1997. PMID: 9309682 Review.
Cited by
- Elevations in the Mitochondrial Matrix Protein Cyclophilin D Correlate With Reduced Parvalbumin Expression in the Prefrontal Cortex of Patients With Schizophrenia.
O'Brien JT, Jalilvand SP, Suji NA, Jupelly RK, Phensy A, Mwirigi JM, Elahi H, Price TJ, Kroener S. O'Brien JT, et al. Schizophr Bull. 2024 Aug 27;50(5):1197-1207. doi: 10.1093/schbul/sbae016. Schizophr Bull. 2024. PMID: 38412332 - Interactions of amyloidogenic proteins with mitochondrial protein import machinery in aging-related neurodegenerative diseases.
Reed AL, Mitchell W, Alexandrescu AT, Alder NN. Reed AL, et al. Front Physiol. 2023 Nov 2;14:1263420. doi: 10.3389/fphys.2023.1263420. eCollection 2023. Front Physiol. 2023. PMID: 38028797 Free PMC article. Review. - The Haves and Have-Nots: The Mitochondrial Permeability Transition Pore across Species.
Frigo E, Tommasin L, Lippe G, Carraro M, Bernardi P. Frigo E, et al. Cells. 2023 May 17;12(10):1409. doi: 10.3390/cells12101409. Cells. 2023. PMID: 37408243 Free PMC article. Review. - Distinct effects of cardiac mitochondrial calcium uniporter inactivation via EMRE deletion in the short and long term.
Chapoy Villanueva H, Sung JH, Stevens JA, Zhang MJ, Nelson PM, Denduluri LS, Feng F, O'Connell TD, Townsend D, Liu JC. Chapoy Villanueva H, et al. J Mol Cell Cardiol. 2023 Aug;181:33-45. doi: 10.1016/j.yjmcc.2023.05.007. Epub 2023 May 23. J Mol Cell Cardiol. 2023. PMID: 37230379 Free PMC article. - Targeting Mitochondria to Control Ageing and Senescence.
Protasoni M, Serrano M. Protasoni M, et al. Pharmaceutics. 2023 Jan 20;15(2):352. doi: 10.3390/pharmaceutics15020352. Pharmaceutics. 2023. PMID: 36839673 Free PMC article. Review.
References
- J Immunol Methods. 1987 Feb 11;96(2):271-8 - PubMed
- Biochem J. 1987 Aug 1;245(3):915-8 - PubMed
- Arch Biochem Biophys. 1988 Sep;265(2):249-57 - PubMed
- Biochem J. 1988 Oct 1;255(1):357-60 - PubMed
- J Biol Chem. 1989 May 15;264(14):7826-30 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous