[16S rRNA reverse transcription-polymerase chain reaction of leptospires] - PubMed (original) (raw)
[Article in Chinese]
- PMID: 7528714
Comparative Study
[16S rRNA reverse transcription-polymerase chain reaction of leptospires]
[Article in Chinese]
W Wu et al. Hua Xi Yi Ke Da Xue Xue Bao. 1994 Jun.
Abstract
We amplified the leptospiral RNAs of Leptospira interrogans serovar lai strain Lai and L. biflexa serovar patoc strain Patoc I, extracted by the method of SiO2-high concentration salt solution (8 mol/L GuHCl) absorption, with the 16S rRNA gene primers of Leptospira interrogans by reverse transcription-polymerase chain reaction (RT-PCR). It demonstrated that the detecting sensitivity by naked eye after electrophoresis could be 100 times higher when we amplified DNA and RNA of leptospires at the same time by RT-PCR.