Epidermal growth factor induces the tyrosine phosphorylation and nuclear translocation of Stat 5 in mouse liver - PubMed (original) (raw)

Epidermal growth factor induces the tyrosine phosphorylation and nuclear translocation of Stat 5 in mouse liver

S Ruff-Jamison et al. Proc Natl Acad Sci U S A. 1995.

Abstract

Intraperitoneal injection of epidermal growth factor into mice results in the appearance of multiple tyrosine-phosphorylated proteins in liver nuclei within minutes after administration. We have previously identified three of these proteins as Stat 1 alpha, Stat 1 beta (p91, p84), and Stat 3 (p89). In the present report we demonstrate that Stat 5 (p92), the recently described prolactin inducible transcription factor detected in mammary glands, is the major tyrosine-phosphorylated protein translocated to the nucleus in mouse liver in response to epidermal growth factor. Furthermore, gel-shift analysis and affinity purification revealed that Stat 5, Stat 1 alpha, and Stat 1 beta specifically bind to the prolactin inducible element upstream of the beta-casein promoter.

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References

    1. Annu Rev Biochem. 1988;57:443-78 - PubMed
    1. Cell. 1990 Apr 20;61(2):203-12 - PubMed
    1. Tumour Biol. 1990;11(5):229-61 - PubMed
    1. Endocr Rev. 1990 Aug;11(3):418-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8477-81 - PubMed

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