CFTR as a cAMP-dependent regulator of sodium channels - PubMed (original) (raw)

. 1995 Aug 11;269(5225):847-50.

doi: 10.1126/science.7543698.

Affiliations

• PMID: 7543698
• DOI: 10.1126/science.7543698

CFTR as a cAMP-dependent regulator of sodium channels

M J Stutts et al. Science. 1995.

Abstract

Cystic fibrosis transmembrane regulator (CFTR), the gene product that is mutated in cystic fibrosis (CF) patients, has a well-recognized function as a cyclic adenosine 3',5'-monophosphate (cAMP)-regulated chloride channel, but this property does not account for the abnormally high basal rate and cAMP sensitivity of sodium ion absorption in CF airway epithelia. Expression of complementary DNAs for rat epithelial Na+ channel (rENaC) alone in Madin Darby canine kidney (MDCK) epithelial cells generated large amiloride-sensitive sodium currents that were stimulated by cAMP, whereas coexpression of human CFTR with rENaC generated smaller basal sodium currents that were inhibited by cAMP. Parallel studies that measured regulation of sodium permeability in fibroblasts showed similar results. In CF airway epithelia, the absence of this second function of CFTR as a cAMP-dependent regulator likely accounts for abnormal sodium transport.

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Comment in

• Regulation of ion channels by ABC transporters that secrete ATP.
  al-Awqati Q. al-Awqati Q. Science. 1995 Aug 11;269(5225):805-6. doi: 10.1126/science.7543697. Science. 1995. PMID: 7543697 Review. No abstract available.

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