An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments - PubMed (original) (raw)

An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments

C Rabouille et al. Cell. 1995.

Free article

Abstract

Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.

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