Cross-talk between the histidine protein kinase VanS and the response regulator PhoB. Characterization and identification of a VanS domain that inhibits activation of PhoB - PubMed (original) (raw)
. 1995 Sep 29;270(39):23143-9.
doi: 10.1074/jbc.270.39.23143.
Affiliations
- PMID: 7559459
- DOI: 10.1074/jbc.270.39.23143
Free article
Cross-talk between the histidine protein kinase VanS and the response regulator PhoB. Characterization and identification of a VanS domain that inhibits activation of PhoB
S L Fisher et al. J Biol Chem. 1995.
Free article
Abstract
VanS is a two-component transmembrane sensory kinase that, together with its response regulator VanR, activates the expression of genes responsible for vancomycin resistance in Enterococcus faecium BM4147. In this report, we demonstrate that the cytoplasmic domain of VanS (including residues Met95 to Ser384) is capable of high level activation (> 500 fold) of the Escherichia coli response regulator PhoB in vivo in the absence of its signaling kinases PhoR, CreC (PhoM), or acetyl phosphate synthesis. In vitro experiments carried out on the purified proteins confirmed that the activation is due to efficient cross-talk between VanS and PhoB, since phospho-VanS catalyzed transfer of its phosphoryl group to PhoB with approximately 90% transfer in 5 min at a 1:4 VanS/PhoB stoichiometry. However, the rate of transfer was at least 100-fold slower than that observed between phospho-VanS and VanR. The in vivo activation of PhoB was used as a reporter system to identify peptide fragments of VanS capable of interfering with activation by VanS(Met95-Ser384), in order to identify an interaction domain. A library of plasmids encoding fragments of VanS(Met95-Ser384) was constructed using transposon mutagenesis, and a subpopulation of these plasmids encoded peptides that interfered with activation of PhoB by VanS(Met95-Ser384). A minimal size fragment (Met95-Ile174) was shown to be both necessary and sufficient for potent inhibition (85%) of this activation.
Similar articles
- Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12.
Haldimann A, Fisher SL, Daniels LL, Walsh CT, Wanner BL. Haldimann A, et al. J Bacteriol. 1997 Sep;179(18):5903-13. doi: 10.1128/jb.179.18.5903-5913.1997. J Bacteriol. 1997. PMID: 9294451 Free PMC article. - Kinetic comparison of the specificity of the vancomycin resistance VanSfor two response regulators, VanR and PhoB.
Fisher SL, Kim SK, Wanner BL, Walsh CT. Fisher SL, et al. Biochemistry. 1996 Apr 16;35(15):4732-40. doi: 10.1021/bi9525435. Biochemistry. 1996. PMID: 8664263 - Gene regulation by phosphate in enteric bacteria.
Wanner BL. Wanner BL. J Cell Biochem. 1993 Jan;51(1):47-54. doi: 10.1002/jcb.240510110. J Cell Biochem. 1993. PMID: 8432742 Review. - Vancomycin resistance VanS/VanR two-component systems.
Hong HJ, Hutchings MI, Buttner MJ; Biotechnology and Biological Sciences Research Council, UK. Hong HJ, et al. Adv Exp Med Biol. 2008;631:200-13. doi: 10.1007/978-0-387-78885-2_14. Adv Exp Med Biol. 2008. PMID: 18792691 Review.
Cited by
- Crosstalk involving two-component systems in Staphylococcus aureus signaling networks.
Ali L, Abdel Aziz MH. Ali L, et al. J Bacteriol. 2024 Apr 18;206(4):e0041823. doi: 10.1128/jb.00418-23. Epub 2024 Mar 8. J Bacteriol. 2024. PMID: 38456702 Free PMC article. Review. - Unsupervised Extraction of Stable Expression Signatures from Public Compendia with an Ensemble of Neural Networks.
Tan J, Doing G, Lewis KA, Price CE, Chen KM, Cady KC, Perchuk B, Laub MT, Hogan DA, Greene CS. Tan J, et al. Cell Syst. 2017 Jul 26;5(1):63-71.e6. doi: 10.1016/j.cels.2017.06.003. Epub 2017 Jul 12. Cell Syst. 2017. PMID: 28711280 Free PMC article. - Revisiting regulation of potassium homeostasis in Escherichia coli: the connection to phosphate limitation.
Schramke H, Laermann V, Tegetmeyer HE, Brachmann A, Jung K, Altendorf K. Schramke H, et al. Microbiologyopen. 2017 Jun;6(3):e00438. doi: 10.1002/mbo3.438. Epub 2017 Jan 17. Microbiologyopen. 2017. PMID: 28097817 Free PMC article. - Prokaryotic Responses to Ammonium and Organic Carbon Reveal Alternative CO2 Fixation Pathways and Importance of Alkaline Phosphatase in the Mesopelagic North Atlantic.
Baltar F, Lundin D, Palovaara J, Lekunberri I, Reinthaler T, Herndl GJ, Pinhassi J. Baltar F, et al. Front Microbiol. 2016 Oct 21;7:1670. doi: 10.3389/fmicb.2016.01670. eCollection 2016. Front Microbiol. 2016. PMID: 27818655 Free PMC article. - Use of restrained molecular dynamics to predict the conformations of phosphorylated receiver domains in two-component signaling systems.
Foster CA, West AH. Foster CA, et al. Proteins. 2017 Jan;85(1):155-176. doi: 10.1002/prot.25207. Epub 2016 Nov 20. Proteins. 2017. PMID: 27802580 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources