A chromatin binding site in the tail domain of nuclear lamins that interacts with core histones - PubMed (original) (raw)
A chromatin binding site in the tail domain of nuclear lamins that interacts with core histones
H Taniura et al. J Cell Biol. 1995 Oct.
Abstract
Interaction of chromatin with the nuclear envelope and lamina is thought to help determine higher order chromosome organization in the interphase nucleus. Previous studies have shown that nuclear lamins bind chromatin directly. Here we have localized a chromatin binding site to the carboxyl-terminal tail domains of both A- and B-type mammalian lamins, and have characterized the biochemical properties of this binding in detail. Recombinant glutathione-S-transferase fusion proteins containing the tail domains of mammalian lamins C, B1, and B2 were analyzed for their ability to associate with rat liver chromatin fragments immobilized on microtiter plate wells. We found that all three lamin tails specifically bind to chromatin with apparent KdS of 120-300 nM. By examining a series of deletion mutants, we have mapped the chromatin binding region of the lamin C tail to amino acids 396-430, a segment immediately adjacent to the rod domain. Furthermore, by analysis of chromatin subfractions, we found that core histones constitute the principal chromatin binding component for the lamin C tail. Through cooperativity, this lamin-histone interaction could be involved in specifying the high avidity attachment of chromatin to the nuclear envelope in vivo.
Similar articles
- The tail domain of lamin Dm0 binds histones H2A and H2B.
Goldberg M, Harel A, Brandeis M, Rechsteiner T, Richmond TJ, Weiss AM, Gruenbaum Y. Goldberg M, et al. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2852-7. doi: 10.1073/pnas.96.6.2852. Proc Natl Acad Sci U S A. 1999. PMID: 10077600 Free PMC article. - Specific and conserved sequences in D. melanogaster and C. elegans lamins and histone H2A mediate the attachment of lamins to chromosomes.
Mattout A, Goldberg M, Tzur Y, Margalit A, Gruenbaum Y. Mattout A, et al. J Cell Sci. 2007 Jan 1;120(Pt 1):77-85. doi: 10.1242/jcs.03325. Epub 2006 Dec 5. J Cell Sci. 2007. PMID: 17148572 - Direct actin binding to A- and B-type lamin tails and actin filament bundling by the lamin A tail.
Simon DN, Zastrow MS, Wilson KL. Simon DN, et al. Nucleus. 2010 May-Jun;1(3):264-72. doi: 10.4161/nucl.1.3.11799. Epub 2010 Mar 11. Nucleus. 2010. PMID: 21327074 Free PMC article. - Lamins and lamin-binding proteins in functional chromatin organization.
Gotzmann J, Foisner R. Gotzmann J, et al. Crit Rev Eukaryot Gene Expr. 1999;9(3-4):257-65. doi: 10.1615/critreveukargeneexpr.v9.i3-4.100. Crit Rev Eukaryot Gene Expr. 1999. PMID: 10651242 Review. - Cytoplasmic control of nuclear assembly.
Collas P. Collas P. Reprod Fertil Dev. 1998;10(7-8):581-92. doi: 10.1071/rd98049. Reprod Fertil Dev. 1998. PMID: 10612464 Review.
Cited by
- The Role of Mechanotransduction in Contact Inhibition of Locomotion and Proliferation.
Nakamura F. Nakamura F. Int J Mol Sci. 2024 Feb 10;25(4):2135. doi: 10.3390/ijms25042135. Int J Mol Sci. 2024. PMID: 38396812 Free PMC article. Review. - Nuclear lamina strain states revealed by intermolecular force biosensor.
Danielsson BE, George Abraham B, Mäntylä E, Cabe JI, Mayer CR, Rekonen A, Ek F, Conway DE, Ihalainen TO. Danielsson BE, et al. Nat Commun. 2023 Jun 30;14(1):3867. doi: 10.1038/s41467-023-39563-6. Nat Commun. 2023. PMID: 37391402 Free PMC article. - Nesprin-1: novel regulator of striated muscle nuclear positioning and mechanotransduction.
De Silva S, Fan Z, Kang B, Shanahan CM, Zhang Q. De Silva S, et al. Biochem Soc Trans. 2023 Jun 28;51(3):1331-1345. doi: 10.1042/BST20221541. Biochem Soc Trans. 2023. PMID: 37171063 Free PMC article. Review. - Close Ties between the Nuclear Envelope and Mammalian Telomeres: Give Me Shelter.
Pennarun G, Picotto J, Bertrand P. Pennarun G, et al. Genes (Basel). 2023 Mar 23;14(4):775. doi: 10.3390/genes14040775. Genes (Basel). 2023. PMID: 37107534 Free PMC article. Review. - Lamin B1 overexpression alters chromatin organization and gene expression.
Kaneshiro JM, Capitanio JS, Hetzer MW. Kaneshiro JM, et al. Nucleus. 2023 Dec;14(1):2202548. doi: 10.1080/19491034.2023.2202548. Nucleus. 2023. PMID: 37071033 Free PMC article.
References
- Biochemistry. 1990 Oct 16;29(41):9551-60 - PubMed
- J Mol Biol. 1989 Aug 5;208(3):405-15 - PubMed
- Curr Opin Cell Biol. 1991 Feb;3(1):82-6 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- Curr Opin Cell Biol. 1994 Feb;6(1):25-33 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases