The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins - PubMed (original) (raw)
The mitochondrial receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins
A Alconada et al. Mol Cell Biol. 1995 Nov.
Abstract
The mitochondrial outer membrane contains import receptors for preproteins and a multisubunit general insertion pore. Several small outer membrane proteins (< 10 kDa) have been identified by their association with receptors or the general insertion pore, yet little is known about their function. Here, we present evidence that the biochemically identified Mom8b and the genetically identified Isp6 are identical. A deletion of Mom8b/Isp6 in Saccharomyces cerevisiae leads to (i) a delay of import of preproteins, (ii) stabilization of preprotein binding to receptors and the general insertion pore, and (iii) destabilization of the interaction between receptors and the general insertion pore. These results suggest that Mom8b supports the cooperativity between receptors and the general insertion pore and facilitates the release of preproteins from import components and thereby promotes efficient transfer of preproteins.
Similar articles
- Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria.
Moczko M, Ehmann B, Gärtner F, Hönlinger A, Schäfer E, Pfanner N. Moczko M, et al. J Biol Chem. 1994 Mar 25;269(12):9045-51. J Biol Chem. 1994. PMID: 8132642 - Tom5 functionally links mitochondrial preprotein receptors to the general import pore.
Dietmeier K, Hönlinger A, Bömer U, Dekker PJ, Eckerskorn C, Lottspeich F, Kübrich M, Pfanner N. Dietmeier K, et al. Nature. 1997 Jul 10;388(6638):195-200. doi: 10.1038/40663. Nature. 1997. PMID: 9217162 - The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane.
Kübrich M, Rassow J, Voos W, Pfanner N, Hönlinger A. Kübrich M, et al. J Biol Chem. 1998 Jun 26;273(26):16374-81. doi: 10.1074/jbc.273.26.16374. J Biol Chem. 1998. PMID: 9632701 - The mitochondrial import machinery for preproteins.
Rehling P, Wiedemann N, Pfanner N, Truscott KN. Rehling P, et al. Crit Rev Biochem Mol Biol. 2001;36(3):291-336. doi: 10.1080/20014091074200. Crit Rev Biochem Mol Biol. 2001. PMID: 11450972 Review. - Protein import into mitochondria.
Paschen SA, Neupert W. Paschen SA, et al. IUBMB Life. 2001 Sep-Nov;52(3-5):101-12. doi: 10.1080/15216540152845894. IUBMB Life. 2001. PMID: 11798021 Review.
Cited by
- Msp1 cooperates with the proteasome for extraction of arrested mitochondrial import intermediates.
Basch M, Wagner M, Rolland S, Carbonell A, Zeng R, Khosravi S, Schmidt A, Aftab W, Imhof A, Wagener J, Conradt B, Wagener N. Basch M, et al. Mol Biol Cell. 2020 Apr 1;31(8):753-767. doi: 10.1091/mbc.E19-06-0329. Epub 2020 Feb 12. Mol Biol Cell. 2020. PMID: 32049577 Free PMC article. - Mitochondrial porin links protein biogenesis to metabolism.
Doan KN, Ellenrieder L, Becker T. Doan KN, et al. Curr Genet. 2019 Aug;65(4):899-903. doi: 10.1007/s00294-019-00965-z. Epub 2019 Apr 3. Curr Genet. 2019. PMID: 30944955 Review. - A conserved endoplasmic reticulum membrane protein complex (EMC) facilitates phospholipid transfer from the ER to mitochondria.
Lahiri S, Chao JT, Tavassoli S, Wong AK, Choudhary V, Young BP, Loewen CJ, Prinz WA. Lahiri S, et al. PLoS Biol. 2014 Oct 14;12(10):e1001969. doi: 10.1371/journal.pbio.1001969. eCollection 2014 Oct. PLoS Biol. 2014. PMID: 25313861 Free PMC article. - Viral product trafficking to mitochondria, mechanisms and roles in pathogenesis.
Williamson CD, DeBiasi RL, Colberg-Poley AM. Williamson CD, et al. Infect Disord Drug Targets. 2012 Feb;12(1):18-37. doi: 10.2174/187152612798994948. Infect Disord Drug Targets. 2012. PMID: 22034933 Free PMC article. Review. - Assembly of the mitochondrial protein import channel: role of Tom5 in two-stage interaction of Tom40 with the SAM complex.
Becker T, Guiard B, Thornton N, Zufall N, Stroud DA, Wiedemann N, Pfanner N. Becker T, et al. Mol Biol Cell. 2010 Sep 15;21(18):3106-13. doi: 10.1091/mbc.E10-06-0518. Epub 2010 Jul 28. Mol Biol Cell. 2010. PMID: 20668160 Free PMC article.
References
- Biochemistry. 1974 Jul 30;13(16):3315-30 - PubMed
- Mol Cell Biol. 1995 Jun;15(6):3382-9 - PubMed
- J Biol Chem. 1982 Nov 10;257(21):13028-33 - PubMed
- Cell. 1987 Jun 19;49(6):815-23 - PubMed
- J Biol Chem. 1987 Jun 5;262(16):7528-36 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases