Selection of novel exon recognition elements from a pool of random sequences - PubMed (original) (raw)
Selection of novel exon recognition elements from a pool of random sequences
H Tian et al. Mol Cell Biol. 1995 Nov.
Abstract
A 20-nucleotide sequence close to the 3' end of the internal exon of a model two-intron, three-exon pre-mRNA (DUP184 [Z. Dominski and R. Kole, J. Biol. Chem. 269:23590-23596, 1994]) was replaced by a random 20-mer, resulting in a pool of pre-mRNAs which, like the initial DUP184 construct, were spliced in vitro by a pathway leading to predominant skipping of the internal exon. The randomized pre-mRNAs were subjected to a selection protocol, resulting in a pool enriched in pre-mRNAs that efficiently included the internal exon. Isolation and sequencing of a number of clones corresponding to the selected pre-mRNAs showed that two classes of sequences were selected from the initial pool. Most abundant among these were sequences with purine tracts similar to those in the recently identified exon-splicing enhancers while a smaller class included sequences lacking discernible purine tracts within the 20-nucleotide region. Splicing of selected pre-mRNAs showed that the purine tracts vary in their ability to promote exon inclusion and, more important, that sequences lacking purine tracts stimulate inclusion of the internal exon as efficiently as their purine-rich counterparts. The latter result indicates the existence of a novel class of exon recognition sequences or splicing enhancers.
Similar articles
- Effects of exon sequences on splicing of model pre-mRNA substrates in vitro.
Dominski Z, Kole R. Dominski Z, et al. Acta Biochim Pol. 1996;43(1):161-73. Acta Biochim Pol. 1996. PMID: 8790721 - Identification of exon sequences involved in splice site selection.
Dominski Z, Kole R. Dominski Z, et al. J Biol Chem. 1994 Sep 23;269(38):23590-6. J Biol Chem. 1994. PMID: 8089127 - Cooperation of pre-mRNA sequence elements in splice site selection.
Dominski Z, Kole R. Dominski Z, et al. Mol Cell Biol. 1992 May;12(5):2108-14. doi: 10.1128/mcb.12.5.2108-2114.1992. Mol Cell Biol. 1992. PMID: 1569943 Free PMC article. - Pre-mRNA secondary structure and the regulation of splicing.
Balvay L, Libri D, Fiszman MY. Balvay L, et al. Bioessays. 1993 Mar;15(3):165-9. doi: 10.1002/bies.950150304. Bioessays. 1993. PMID: 8489523 Review. - Splicing in action: assessing disease causing sequence changes.
Baralle D, Baralle M. Baralle D, et al. J Med Genet. 2005 Oct;42(10):737-48. doi: 10.1136/jmg.2004.029538. J Med Genet. 2005. PMID: 16199547 Free PMC article. Review.
Cited by
- Systematic characterization of short intronic splicing-regulatory elements in SMN2 pre-mRNA.
Gao Y, Lin KT, Jiang T, Yang Y, Rahman MA, Gong S, Bai J, Wang L, Sun J, Sheng L, Krainer AR, Hua Y. Gao Y, et al. Nucleic Acids Res. 2022 Jan 25;50(2):731-749. doi: 10.1093/nar/gkab1280. Nucleic Acids Res. 2022. PMID: 35018432 Free PMC article. - Mutational bias and the protein code shape the evolution of splicing enhancers.
Rong S, Buerer L, Rhine CL, Wang J, Cygan KJ, Fairbrother WG. Rong S, et al. Nat Commun. 2020 Jun 5;11(1):2845. doi: 10.1038/s41467-020-16673-z. Nat Commun. 2020. PMID: 32504065 Free PMC article. - Context-dependent control of alternative splicing by RNA-binding proteins.
Fu XD, Ares M Jr. Fu XD, et al. Nat Rev Genet. 2014 Oct;15(10):689-701. doi: 10.1038/nrg3778. Epub 2014 Aug 12. Nat Rev Genet. 2014. PMID: 25112293 Free PMC article. Review. - Intronic splicing enhancers, cognate splicing factors and context-dependent regulation rules.
Wang Y, Ma M, Xiao X, Wang Z. Wang Y, et al. Nat Struct Mol Biol. 2012 Oct;19(10):1044-52. doi: 10.1038/nsmb.2377. Epub 2012 Sep 16. Nat Struct Mol Biol. 2012. PMID: 22983564 Free PMC article. - Splicing of internal large exons is defined by novel cis-acting sequence elements.
Bolisetty MT, Beemon KL. Bolisetty MT, et al. Nucleic Acids Res. 2012 Oct;40(18):9244-54. doi: 10.1093/nar/gks652. Epub 2012 Jul 11. Nucleic Acids Res. 2012. PMID: 22790982 Free PMC article.
References
- Cell. 1984 Oct;38(3):731-6 - PubMed
- RNA. 1995 Mar;1(1):21-35 - PubMed
- Proc Natl Acad Sci U S A. 1986 Feb;83(4):927-31 - PubMed
- Cell. 1986 Aug 29;46(5):681-90 - PubMed
- J Biol Chem. 1987 Aug 15;262(23):11284-91 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous