Growth-dependent translation of IGF-II mRNA by a rapamycin-sensitive pathway - PubMed (original) (raw)
. 1995 Sep 28;377(6547):358-62.
doi: 10.1038/377358a0.
Affiliations
- PMID: 7566093
- DOI: 10.1038/377358a0
Growth-dependent translation of IGF-II mRNA by a rapamycin-sensitive pathway
F C Nielsen et al. Nature. 1995.
Abstract
Insulin-like growth factor (IGF)-II is important for fetal growth and development. The human IGF-II gene generates multiple mature transcripts with different 5' untranslated regions (5'UTRs) but identical coding regions and 3'UTRs. We have previously shown that a minor 4.8-kilobase messenger RNA was engaged in the synthesis of preproIGF-II, and a major 6.0-kb mRNA was untranslated and stored in a 100S ribonucleoprotein particle. Here we demonstrate that the 6.0-kb mRNA is selectively mobilized and translated in dispersed exponentially growing cells. Translational activation is prevented by rapamycin and mimicked by anisomycin, which suggests that translation of the 6.0-kb mRNA is regulated by the p70S6k/85S6k kinase signalling pathway. Therefore, the minor 4.8-kb mRNA generates a constitutive production of prepro-IGF-II, whereas the major 6.0-kb mRNA provides a post-transcriptionally regulated species.
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