rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion - PubMed (original) (raw)
rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion
A E Ting et al. Proc Natl Acad Sci U S A. 1995.
Abstract
Many of the molecules necessary for neurotransmission are homologous to proteins involved in the Golgi-to-plasma membrane stage of the yeast secretory pathway. Of 15 genes known to be essential for the later stages of vesicle trafficking in yeast, 7 have no identified mammalian homologs. These include the yeast SEC6, SEC8, and SEC15 genes, whose products are constituents of a 19.5S particle that interacts with the GTP-binding protein Sec4p. Here we report the sequences of rSec6 and rSec8, rat homologs of Sec6p and Sec8p. The rSec6 cDNA is predicted to encode an 87-kDa protein with 22% amino acid identity to Sec6p, and the rSec8 cDNA is predicted to encode a 110-kDa protein which is 20% identical to Sec8p. Northern blot analysis indicates that rSec6 and rSec8 are expressed in similar tissues. Immunodetection reveals that rSec8 is part of a soluble 17S particle in brain. COS cell cotransfection studies demonstrate that rSec8 colocalizes with the GTP-binding protein Rab3a and syntaxin 1a, two proteins involved in synaptic vesicle docking and fusion at the presynaptic terminal. These data suggest that rSec8 is a component of a high molecular weight complex which may participate in the regulation of vesicle docking and fusion in brain.
References
- Cell. 1993 Nov 5;75(3):409-18 - PubMed
- EMBO J. 1993 Nov;12(11):4095-104 - PubMed
- J Mol Biol. 1975 Oct 25;98(2):293-304 - PubMed
- Mol Cell Biol. 1991 Feb;11(2):872-85 - PubMed
- Methods Enzymol. 1991;194:239-51 - PubMed
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